Purification of DNA repair protein complexes from mammalian cells
Cells possess multiple DNA repair pathways to tackle a variety of DNA lesions. Often, DNA repair proteins function as large protein complexes. Here, we describe a protocol to purify DNA repair protein complexes from nuclei of mammalian cells. The method permits purification of protein complexes cont...
| Main Authors: | , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
Elsevier
2021
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| _version_ | 1797104396743475200 |
|---|---|
| author | Liang, C-C Cohn, MA |
| author_facet | Liang, C-C Cohn, MA |
| author_sort | Liang, C-C |
| collection | OXFORD |
| description | Cells possess multiple DNA repair pathways to tackle a variety of DNA lesions. Often,
DNA repair proteins function as large protein complexes. Here, we describe a protocol
to purify DNA repair protein complexes from nuclei of mammalian cells. The method
permits purification of protein complexes containing stable as well as transiently
associated proteins, which subsequently can be identified by mass spectrometry
analysis. This protocol can be applied to uncover the functions and mechanism of DNA
repair pathways. |
| first_indexed | 2024-03-07T06:33:16Z |
| format | Journal article |
| id | oxford-uuid:f6c333e2-f4c0-4999-bcdf-a6391f07dac9 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:33:16Z |
| publishDate | 2021 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oxford-uuid:f6c333e2-f4c0-4999-bcdf-a6391f07dac92022-03-27T12:37:22ZPurification of DNA repair protein complexes from mammalian cellsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:f6c333e2-f4c0-4999-bcdf-a6391f07dac9EnglishSymplectic ElementsElsevier2021Liang, C-CCohn, MACells possess multiple DNA repair pathways to tackle a variety of DNA lesions. Often, DNA repair proteins function as large protein complexes. Here, we describe a protocol to purify DNA repair protein complexes from nuclei of mammalian cells. The method permits purification of protein complexes containing stable as well as transiently associated proteins, which subsequently can be identified by mass spectrometry analysis. This protocol can be applied to uncover the functions and mechanism of DNA repair pathways. |
| spellingShingle | Liang, C-C Cohn, MA Purification of DNA repair protein complexes from mammalian cells |
| title | Purification of DNA repair protein complexes from mammalian cells |
| title_full | Purification of DNA repair protein complexes from mammalian cells |
| title_fullStr | Purification of DNA repair protein complexes from mammalian cells |
| title_full_unstemmed | Purification of DNA repair protein complexes from mammalian cells |
| title_short | Purification of DNA repair protein complexes from mammalian cells |
| title_sort | purification of dna repair protein complexes from mammalian cells |
| work_keys_str_mv | AT liangcc purificationofdnarepairproteincomplexesfrommammaliancells AT cohnma purificationofdnarepairproteincomplexesfrommammaliancells |