Quantitative comparison of different fluorescent protein couples for fast FRET-FLIM acquisition.
The fluorescent-protein based fluorescence resonance energy transfer (FRET) approach is a powerful method for quantifying protein-protein interactions in living cells, especially when combined with fluorescence lifetime imaging microscopy (FLIM). To compare the performance of different FRET couples...
Main Authors: | , , , , , |
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Format: | Journal article |
Language: | English |
Published: |
2009
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