Specific transcriptional pausing activates polyadenylation in a coupled in vitro system.
We have developed a coupled in vitro transcription-polyadenylation system to investigate RNA polymerase II (Pol II) termination, which depends on active polyadenylation of the nascent RNA. Specific G-rich sequences originally identified as binding sites for the transcription factor MAZ both pause Po...
| Main Authors: | , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1999
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