An efficient purification system for native minichromosome from Saccharomyces cerevisiae.

We have recently established a system for purifying minichromosomes in a native state from Saccharomyces cerevisiae. This system is extremely efficient, and a single-step purification yields samples with sufficient purity and quantity for mass spectrometry (MS) analysis of histones and non-histone p...

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Bibliographic Details
Main Authors: Unnikrishnan, A, Akiyoshi, B, Biggins, S, Tsukiyama, T
Format: Journal article
Language:English
Published: 2012