An efficient purification system for native minichromosome from Saccharomyces cerevisiae.
We have recently established a system for purifying minichromosomes in a native state from Saccharomyces cerevisiae. This system is extremely efficient, and a single-step purification yields samples with sufficient purity and quantity for mass spectrometry (MS) analysis of histones and non-histone p...
Hauptverfasser: | Unnikrishnan, A, Akiyoshi, B, Biggins, S, Tsukiyama, T |
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Format: | Journal article |
Sprache: | English |
Veröffentlicht: |
2012
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