p53-specific CD8+ T-cell responses in individuals with cutaneous squamous cell carcinoma.
BACKGROUND: Systemic immunosuppression is a significant risk factor for cutaneous squamous cell carcinoma (SCC). p53 is mutated and overexpressed in up to 90% of cutaneous SCC lesions. Despite considerable evidence that the immune response is important in the control of cutaneous SCC, there are no s...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2005
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| _version_ | 1797105456715399168 |
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| author | Black, A Bailey, A Jones, L Turner, R Hollowood, K Ogg, G |
| author_facet | Black, A Bailey, A Jones, L Turner, R Hollowood, K Ogg, G |
| author_sort | Black, A |
| collection | OXFORD |
| description | BACKGROUND: Systemic immunosuppression is a significant risk factor for cutaneous squamous cell carcinoma (SCC). p53 is mutated and overexpressed in up to 90% of cutaneous SCC lesions. Despite considerable evidence that the immune response is important in the control of cutaneous SCC, there are no studies documenting potential tumour-associated antigens. OBJECTIVES: We tested the hypothesis that individuals with cutaneous SCC have functional circulating CD8+ T cells specific for p53. METHODS: Interferon-gamma immunosorbent assays were used to screen peripheral blood mononuclear cells for reactivity to six p53-derived HLA-A*0201-restricted epitopes from HLA-A*0201-positive patients and controls. RESULTS: We observed significantly elevated frequencies of p53-specific CD8+ T cells in seven of 26 individuals with cutaneous SCC and in one of 10 controls. The degree of lymphocytic infiltrate significantly correlated with the frequency of CD8+ T cells specific for p53 epitopes, but not with control epitopes. CONCLUSIONS: Overall, these data suggest that p53 may represent a target for CD8+ T cells in a proportion of individuals with cutaneous SCC. |
| first_indexed | 2024-03-07T06:47:50Z |
| format | Journal article |
| id | oxford-uuid:fb7620fa-9ccc-48ee-a133-3980333f21ad |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:47:50Z |
| publishDate | 2005 |
| record_format | dspace |
| spelling | oxford-uuid:fb7620fa-9ccc-48ee-a133-3980333f21ad2022-03-27T13:13:59Zp53-specific CD8+ T-cell responses in individuals with cutaneous squamous cell carcinoma.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:fb7620fa-9ccc-48ee-a133-3980333f21adEnglishSymplectic Elements at Oxford2005Black, ABailey, AJones, LTurner, RHollowood, KOgg, GBACKGROUND: Systemic immunosuppression is a significant risk factor for cutaneous squamous cell carcinoma (SCC). p53 is mutated and overexpressed in up to 90% of cutaneous SCC lesions. Despite considerable evidence that the immune response is important in the control of cutaneous SCC, there are no studies documenting potential tumour-associated antigens. OBJECTIVES: We tested the hypothesis that individuals with cutaneous SCC have functional circulating CD8+ T cells specific for p53. METHODS: Interferon-gamma immunosorbent assays were used to screen peripheral blood mononuclear cells for reactivity to six p53-derived HLA-A*0201-restricted epitopes from HLA-A*0201-positive patients and controls. RESULTS: We observed significantly elevated frequencies of p53-specific CD8+ T cells in seven of 26 individuals with cutaneous SCC and in one of 10 controls. The degree of lymphocytic infiltrate significantly correlated with the frequency of CD8+ T cells specific for p53 epitopes, but not with control epitopes. CONCLUSIONS: Overall, these data suggest that p53 may represent a target for CD8+ T cells in a proportion of individuals with cutaneous SCC. |
| spellingShingle | Black, A Bailey, A Jones, L Turner, R Hollowood, K Ogg, G p53-specific CD8+ T-cell responses in individuals with cutaneous squamous cell carcinoma. |
| title | p53-specific CD8+ T-cell responses in individuals with cutaneous squamous cell carcinoma. |
| title_full | p53-specific CD8+ T-cell responses in individuals with cutaneous squamous cell carcinoma. |
| title_fullStr | p53-specific CD8+ T-cell responses in individuals with cutaneous squamous cell carcinoma. |
| title_full_unstemmed | p53-specific CD8+ T-cell responses in individuals with cutaneous squamous cell carcinoma. |
| title_short | p53-specific CD8+ T-cell responses in individuals with cutaneous squamous cell carcinoma. |
| title_sort | p53 specific cd8 t cell responses in individuals with cutaneous squamous cell carcinoma |
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