A high-content screening platform utilizing polarization anisotropy and FLIM microscopy

An automated high-content screening microscope has been developed which uses fluorescence anisotropy imaging and fluorescence lifetime microscopy to identify Förster resonant energy transfer between eGFP and mRPF1 in drug screening assays. A wide-field polarization resolved imager is used to simulta...

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Bibliographic Details
Main Authors: Matthews, DR, Ameer-Beg, S, Barber, P, Pierce, G, Newman, R, Vojnovic, B, Carlin, L, Keppler, MD, Ng, T, Suhling, K, Irving, M
Format: Journal article
Language:English
Published: 2008