Induced sputum and bronchial mucosal expression of interleukin-13 is not increased in chronic obstructive pulmonary disease.
BACKGROUND: The Th2 cytokine interleukin-13 (IL-13) has been implicated in the pathogenesis of chronic obstructive pulmonary disease (COPD). We sought to examine IL-13 expression in COPD subjects in induced sputum and bronchus specimens. We hypothesized that inflammatory cells expressing IL-13 local...
| Huvudupphovsmän: | , , , , , , , |
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| Materialtyp: | Journal article |
| Språk: | English |
| Publicerad: |
2008
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| _version_ | 1826306765627588608 |
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| author | Saha, S Mistry, V Siva, R Parker, D May, R Bradding, P Pavord, I Brightling, C |
| author_facet | Saha, S Mistry, V Siva, R Parker, D May, R Bradding, P Pavord, I Brightling, C |
| author_sort | Saha, S |
| collection | OXFORD |
| description | BACKGROUND: The Th2 cytokine interleukin-13 (IL-13) has been implicated in the pathogenesis of chronic obstructive pulmonary disease (COPD). We sought to examine IL-13 expression in COPD subjects in induced sputum and bronchus specimens. We hypothesized that inflammatory cells expressing IL-13 localize to the airway smooth muscle bundle and bronchial glands. METHODS: Interleukin-13 was measured in sputum samples from subjects with COPD (n = 34) across a range of severity (Global initiative for chronic Obstructive Lung Disease 2-4) and controls (n = 14) using ELISA. IL-13+ cells and inflammatory cells were enumerated within surgically resected proximal airway using immunohistochemical techniques from subjects with COPD (n = 10), smoking (n = 10) and nonsmoking controls (n = 8). RESULTS: Sputum IL-13 was measurable in only 6/34 subjects with COPD and was not found in the smoking or nonsmoking control subjects. In subjects with COPD and controls there was a paucity of IL-13+ cells. The distribution of inflammatory cells within different airway compartments was similar in COPD and controls except for an increase in CD3(+) lymphocytes within bronchial glands in COPD (P = 0.04). CONCLUSIONS: Our findings do not support a role for IL-13 in COPD. However, the tissue localization of inflammatory cells to airway compartments, particularly the increase of T cells in glands in COPD may be important in disease. |
| first_indexed | 2024-03-07T06:52:53Z |
| format | Journal article |
| id | oxford-uuid:fd1fe26a-3a8d-453e-a36b-f2aee626b87b |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:52:53Z |
| publishDate | 2008 |
| record_format | dspace |
| spelling | oxford-uuid:fd1fe26a-3a8d-453e-a36b-f2aee626b87b2022-03-27T13:26:31ZInduced sputum and bronchial mucosal expression of interleukin-13 is not increased in chronic obstructive pulmonary disease.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:fd1fe26a-3a8d-453e-a36b-f2aee626b87bEnglishSymplectic Elements at Oxford2008Saha, SMistry, VSiva, RParker, DMay, RBradding, PPavord, IBrightling, CBACKGROUND: The Th2 cytokine interleukin-13 (IL-13) has been implicated in the pathogenesis of chronic obstructive pulmonary disease (COPD). We sought to examine IL-13 expression in COPD subjects in induced sputum and bronchus specimens. We hypothesized that inflammatory cells expressing IL-13 localize to the airway smooth muscle bundle and bronchial glands. METHODS: Interleukin-13 was measured in sputum samples from subjects with COPD (n = 34) across a range of severity (Global initiative for chronic Obstructive Lung Disease 2-4) and controls (n = 14) using ELISA. IL-13+ cells and inflammatory cells were enumerated within surgically resected proximal airway using immunohistochemical techniques from subjects with COPD (n = 10), smoking (n = 10) and nonsmoking controls (n = 8). RESULTS: Sputum IL-13 was measurable in only 6/34 subjects with COPD and was not found in the smoking or nonsmoking control subjects. In subjects with COPD and controls there was a paucity of IL-13+ cells. The distribution of inflammatory cells within different airway compartments was similar in COPD and controls except for an increase in CD3(+) lymphocytes within bronchial glands in COPD (P = 0.04). CONCLUSIONS: Our findings do not support a role for IL-13 in COPD. However, the tissue localization of inflammatory cells to airway compartments, particularly the increase of T cells in glands in COPD may be important in disease. |
| spellingShingle | Saha, S Mistry, V Siva, R Parker, D May, R Bradding, P Pavord, I Brightling, C Induced sputum and bronchial mucosal expression of interleukin-13 is not increased in chronic obstructive pulmonary disease. |
| title | Induced sputum and bronchial mucosal expression of interleukin-13 is not increased in chronic obstructive pulmonary disease. |
| title_full | Induced sputum and bronchial mucosal expression of interleukin-13 is not increased in chronic obstructive pulmonary disease. |
| title_fullStr | Induced sputum and bronchial mucosal expression of interleukin-13 is not increased in chronic obstructive pulmonary disease. |
| title_full_unstemmed | Induced sputum and bronchial mucosal expression of interleukin-13 is not increased in chronic obstructive pulmonary disease. |
| title_short | Induced sputum and bronchial mucosal expression of interleukin-13 is not increased in chronic obstructive pulmonary disease. |
| title_sort | induced sputum and bronchial mucosal expression of interleukin 13 is not increased in chronic obstructive pulmonary disease |
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