Open science discovery of potent noncovalent SARS-CoV-2 main protease inhibitors
<strong>INTRODUCTION<br></strong> COVID-19 became a global pandemic partially as a result of the lack of easily deployable, broad-spectrum oral antivirals, which complicated its containment. Even endemically, and with effective vaccinations, it will continue to cause acute disease...
Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
---|---|
Other Authors: | |
Format: | Journal article |
Language: | English |
Published: |
American Association for the Advancement of Science
2023
|
_version_ | 1797112089640173568 |
---|---|
author | Boby, ML Fearon, D Ferla, M Filep, M Koekemoer, L Robinson, MC Chodera, JD Lee, AA London, N von Delft, A von Delft, F Achdout, H Aimon, A Alonzi, DS Arbon, R Aschenbrenner, JC Balcomb, BH Bar-David, E Barr, H Ben-Shmuel, A Bennett, J Bilenko, VA Borden, B Boulet, P Bowman, GR Brewitz, L Brun, J Bvnbs, S Calmiano, M Carbery, A Carney, DW Cattermole, E Chang, E Chernyshenko, E Clyde, A Coffland, JE Cohen, G Cole, JC Contini, A Cox, L Croll, TI Cvitkovic, M De Jonghe, S Dias, A Donckers, K Dotson, DL Douangamath, A Duberstein, S Dudgeon, T Fairhead, M Taylor, JC Zitzmann, N |
author2 | COVID Moonshot Consortium |
author_facet | COVID Moonshot Consortium Boby, ML Fearon, D Ferla, M Filep, M Koekemoer, L Robinson, MC Chodera, JD Lee, AA London, N von Delft, A von Delft, F Achdout, H Aimon, A Alonzi, DS Arbon, R Aschenbrenner, JC Balcomb, BH Bar-David, E Barr, H Ben-Shmuel, A Bennett, J Bilenko, VA Borden, B Boulet, P Bowman, GR Brewitz, L Brun, J Bvnbs, S Calmiano, M Carbery, A Carney, DW Cattermole, E Chang, E Chernyshenko, E Clyde, A Coffland, JE Cohen, G Cole, JC Contini, A Cox, L Croll, TI Cvitkovic, M De Jonghe, S Dias, A Donckers, K Dotson, DL Douangamath, A Duberstein, S Dudgeon, T Fairhead, M Taylor, JC Zitzmann, N |
author_sort | Boby, ML |
collection | OXFORD |
description | <strong>INTRODUCTION<br></strong>
COVID-19 became a global pandemic partially as a result of the lack of easily deployable, broad-spectrum oral antivirals, which complicated its containment. Even endemically, and with effective vaccinations, it will continue to cause acute disease, death, and long-term sequelae globally unless there are accessible treatments. COVID-19 is not an isolated event but instead is the latest example of a viral pandemic threat to human health. Therefore, antiviral discovery and development should be a key pillar of pandemic preparedness efforts.
<br><strong>RATIONALE<br></strong>
One route to accelerate antiviral drug discovery is the establishment of open knowledge bases, the development of effective technology infrastructures, and the discovery of multiple potent antivirals suitable as starting points for the development of therapeutics. In this work, we report the results of the COVID Moonshot—a fully open science, crowdsourced, and structure-enabled drug discovery campaign—against the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) main protease (Mpro). This collaboration may serve as a roadmap for the potential development of future antivirals.
<br><strong>RESULTS<br></strong>
On the basis of the results of a crystallographic fragment screen, we crowdsourced design ideas to progress from fragment to lead compounds. The crowdsourcing strategy yielded several key compounds along the optimization trajectory, including the starting compound of what became the primary lead series. Three additional chemically distinct lead series were also explored, spanning a diversity of chemotypes.
The collaborative and highly automated nature of the COVID Moonshot Consortium resulted in >18,000 compound designs, >2400 synthesized compounds, >490 ligand-bound x-ray structures, >22,000 alchemical free-energy calculations, and >10,000 biochemical measurements—all of which were made publicly available in real time. The recently approved antiviral ensitrelvir was identified in part based on crystallographic data from the COVID Moonshot Consortium.<br>
This campaign led to the discovery of a potent [median inhibitory concentration (IC50) = 37 ± 2 nM] and differentiated (noncovalent and nonpeptidic) lead compound that also exhibited potent cellular activity, with a median effective concentration (EC50) of 64 nM in A549-ACE2-TMPRSS2 cells and 126 nM in HeLa-ACE2 cells without measurable cytotoxicity. Although the pharmacokinetics of the reported compound is not yet optimal for therapeutic development, it is a promising starting point for further antiviral discovery and development.
<br><strong>CONCLUSION<br></strong>
The success of the COVID Moonshot project in producing potent antivirals, building open knowledge bases, accelerating external discovery efforts, and functioning as a useful information-exchange hub is an example of the potential effectiveness of open science antiviral discovery programs. The open science, patent-free nature of the project enabled a large number of collaborators to provide in-kind support, including synthesis, assays, and in vitro and in vivo experiments. By making all data immediately available and ensuring that all compounds are purchasable from Enamine without the need for materials transfer agreements, we aim to accelerate research globally along parallel tracks. In the process, we generated a detailed map of the structural plasticity of Mpro, extensive structure-activity relationships for multiple chemotypes, and a wealth of biochemical activity data to spur further research into antivirals and discovery methodologies. We hope that this can serve as an alternative model for antiviral discovery and future pandemic preparedness.<br>
Further, the project also showcases the role of machine learning, computational chemistry, and high-throughput structural biology as force multipliers in drug design. Artificial intelligence and machine learning algorithms help accelerate chemical synthesis while balancing multiple competing molecular properties. The design-make-test-analyze cycle was accelerated by these algorithms combined with planetary-scale biomolecular simulations of protein-ligand interactions and rapid structure determination. |
first_indexed | 2024-03-07T08:19:30Z |
format | Journal article |
id | oxford-uuid:ff7373b3-5e03-47f3-ba8b-9fb5b2c29864 |
institution | University of Oxford |
language | English |
last_indexed | 2024-03-07T08:19:30Z |
publishDate | 2023 |
publisher | American Association for the Advancement of Science |
record_format | dspace |
spelling | oxford-uuid:ff7373b3-5e03-47f3-ba8b-9fb5b2c298642024-01-17T10:42:13ZOpen science discovery of potent noncovalent SARS-CoV-2 main protease inhibitorsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:ff7373b3-5e03-47f3-ba8b-9fb5b2c29864EnglishSymplectic ElementsAmerican Association for the Advancement of Science2023Boby, MLFearon, DFerla, MFilep, MKoekemoer, LRobinson, MCChodera, JDLee, AALondon, Nvon Delft, Avon Delft, FAchdout, HAimon, AAlonzi, DSArbon, RAschenbrenner, JCBalcomb, BHBar-David, EBarr, HBen-Shmuel, ABennett, JBilenko, VABorden, BBoulet, PBowman, GRBrewitz, LBrun, JBvnbs, SCalmiano, MCarbery, ACarney, DWCattermole, EChang, EChernyshenko, EClyde, ACoffland, JECohen, GCole, JCContini, ACox, LCroll, TICvitkovic, MDe Jonghe, SDias, ADonckers, KDotson, DLDouangamath, ADuberstein, SDudgeon, TFairhead, MTaylor, JCZitzmann, NCOVID Moonshot Consortium<strong>INTRODUCTION<br></strong> COVID-19 became a global pandemic partially as a result of the lack of easily deployable, broad-spectrum oral antivirals, which complicated its containment. Even endemically, and with effective vaccinations, it will continue to cause acute disease, death, and long-term sequelae globally unless there are accessible treatments. COVID-19 is not an isolated event but instead is the latest example of a viral pandemic threat to human health. Therefore, antiviral discovery and development should be a key pillar of pandemic preparedness efforts. <br><strong>RATIONALE<br></strong> One route to accelerate antiviral drug discovery is the establishment of open knowledge bases, the development of effective technology infrastructures, and the discovery of multiple potent antivirals suitable as starting points for the development of therapeutics. In this work, we report the results of the COVID Moonshot—a fully open science, crowdsourced, and structure-enabled drug discovery campaign—against the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) main protease (Mpro). This collaboration may serve as a roadmap for the potential development of future antivirals. <br><strong>RESULTS<br></strong> On the basis of the results of a crystallographic fragment screen, we crowdsourced design ideas to progress from fragment to lead compounds. The crowdsourcing strategy yielded several key compounds along the optimization trajectory, including the starting compound of what became the primary lead series. Three additional chemically distinct lead series were also explored, spanning a diversity of chemotypes. The collaborative and highly automated nature of the COVID Moonshot Consortium resulted in >18,000 compound designs, >2400 synthesized compounds, >490 ligand-bound x-ray structures, >22,000 alchemical free-energy calculations, and >10,000 biochemical measurements—all of which were made publicly available in real time. The recently approved antiviral ensitrelvir was identified in part based on crystallographic data from the COVID Moonshot Consortium.<br> This campaign led to the discovery of a potent [median inhibitory concentration (IC50) = 37 ± 2 nM] and differentiated (noncovalent and nonpeptidic) lead compound that also exhibited potent cellular activity, with a median effective concentration (EC50) of 64 nM in A549-ACE2-TMPRSS2 cells and 126 nM in HeLa-ACE2 cells without measurable cytotoxicity. Although the pharmacokinetics of the reported compound is not yet optimal for therapeutic development, it is a promising starting point for further antiviral discovery and development. <br><strong>CONCLUSION<br></strong> The success of the COVID Moonshot project in producing potent antivirals, building open knowledge bases, accelerating external discovery efforts, and functioning as a useful information-exchange hub is an example of the potential effectiveness of open science antiviral discovery programs. The open science, patent-free nature of the project enabled a large number of collaborators to provide in-kind support, including synthesis, assays, and in vitro and in vivo experiments. By making all data immediately available and ensuring that all compounds are purchasable from Enamine without the need for materials transfer agreements, we aim to accelerate research globally along parallel tracks. In the process, we generated a detailed map of the structural plasticity of Mpro, extensive structure-activity relationships for multiple chemotypes, and a wealth of biochemical activity data to spur further research into antivirals and discovery methodologies. We hope that this can serve as an alternative model for antiviral discovery and future pandemic preparedness.<br> Further, the project also showcases the role of machine learning, computational chemistry, and high-throughput structural biology as force multipliers in drug design. Artificial intelligence and machine learning algorithms help accelerate chemical synthesis while balancing multiple competing molecular properties. The design-make-test-analyze cycle was accelerated by these algorithms combined with planetary-scale biomolecular simulations of protein-ligand interactions and rapid structure determination. |
spellingShingle | Boby, ML Fearon, D Ferla, M Filep, M Koekemoer, L Robinson, MC Chodera, JD Lee, AA London, N von Delft, A von Delft, F Achdout, H Aimon, A Alonzi, DS Arbon, R Aschenbrenner, JC Balcomb, BH Bar-David, E Barr, H Ben-Shmuel, A Bennett, J Bilenko, VA Borden, B Boulet, P Bowman, GR Brewitz, L Brun, J Bvnbs, S Calmiano, M Carbery, A Carney, DW Cattermole, E Chang, E Chernyshenko, E Clyde, A Coffland, JE Cohen, G Cole, JC Contini, A Cox, L Croll, TI Cvitkovic, M De Jonghe, S Dias, A Donckers, K Dotson, DL Douangamath, A Duberstein, S Dudgeon, T Fairhead, M Taylor, JC Zitzmann, N Open science discovery of potent noncovalent SARS-CoV-2 main protease inhibitors |
title | Open science discovery of potent noncovalent SARS-CoV-2 main protease inhibitors |
title_full | Open science discovery of potent noncovalent SARS-CoV-2 main protease inhibitors |
title_fullStr | Open science discovery of potent noncovalent SARS-CoV-2 main protease inhibitors |
title_full_unstemmed | Open science discovery of potent noncovalent SARS-CoV-2 main protease inhibitors |
title_short | Open science discovery of potent noncovalent SARS-CoV-2 main protease inhibitors |
title_sort | open science discovery of potent noncovalent sars cov 2 main protease inhibitors |
work_keys_str_mv | AT bobyml opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT fearond opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT ferlam opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT filepm opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT koekemoerl opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT robinsonmc opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT choderajd opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT leeaa opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT londonn opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT vondelfta opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT vondelftf opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT achdouth opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT aimona opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT alonzids opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT arbonr opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT aschenbrennerjc opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT balcombbh opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT bardavide opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT barrh opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT benshmuela opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT bennettj opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT bilenkova opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT bordenb opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT bouletp opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT bowmangr opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT brewitzl opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT brunj opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT bvnbss opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT calmianom opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT carberya opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT carneydw opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT cattermolee opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT change opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT chernyshenkoe opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT clydea opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT cofflandje opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT coheng opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT colejc opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT continia opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT coxl opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT crollti opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT cvitkovicm opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT dejonghes opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT diasa opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT donckersk opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT dotsondl opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT douangamatha opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT dubersteins opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT dudgeont opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT fairheadm opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT taylorjc opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors AT zitzmannn opensciencediscoveryofpotentnoncovalentsarscov2mainproteaseinhibitors |