Preliminary study of in vitro propagation of a selected high yielding clone of kacip fatimah (Labisia pumila VAR. alata)

Kacip Fatimah (Labisia pumila), an undershrub herb from the Myrsinaceae family, is traditionally used as medicine, particularly by Malay women during post-natal care. The development of L. pumila as a medicinal herb has led to its commercialization and increasing demand. To ensure its sustainable...

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Main Authors: Syafiqah Nabilah, S.B., Farah Fazwa, M.A., Nor Hasnida, H., Siti Suhaila, A.R., Norhayati, S., Nazirah, A., Muhammad Fuad, Y., Haliza, I., Mohamad, O.
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2015
Online Access:http://journalarticle.ukm.my/8717/1/44_1_19.pdf
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author Syafiqah Nabilah, S.B.
Farah Fazwa, M.A.
Nor Hasnida, H.
Siti Suhaila, A.R.
Norhayati, S.
Nazirah, A.
Muhammad Fuad, Y.
Haliza, I.
Mohamad, O.
author_facet Syafiqah Nabilah, S.B.
Farah Fazwa, M.A.
Nor Hasnida, H.
Siti Suhaila, A.R.
Norhayati, S.
Nazirah, A.
Muhammad Fuad, Y.
Haliza, I.
Mohamad, O.
author_sort Syafiqah Nabilah, S.B.
collection UKM
description Kacip Fatimah (Labisia pumila), an undershrub herb from the Myrsinaceae family, is traditionally used as medicine, particularly by Malay women during post-natal care. The development of L. pumila as a medicinal herb has led to its commercialization and increasing demand. To ensure its sustainable supply, the propagation of kacip Fatimah on a large scale such as through tissue culture needs to be explored. In this study, the leaves of a selected high yielding clone of kacip Fatimah, clone AA28, were used as explants. After surface sterilization, the explants were cultured on MS basal medium containing 0.5 mg/l NAA with different concentrations of BAP ranging from 0.05 to 0.15 mg/l for shoot induction. The explants cultured in MS medium containing 0.5 mg/l NAA in combination with 0.05 mg/l BAP produced the highest mean number of shoots per explants (10 ± 0.1). The shoot multiplication study is in progress and will be reported in a future paper.
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spelling ukm.eprints-87172016-12-14T06:47:59Z http://journalarticle.ukm.my/8717/ Preliminary study of in vitro propagation of a selected high yielding clone of kacip fatimah (Labisia pumila VAR. alata) Syafiqah Nabilah, S.B. Farah Fazwa, M.A. Nor Hasnida, H. Siti Suhaila, A.R. Norhayati, S. Nazirah, A. Muhammad Fuad, Y. Haliza, I. Mohamad, O. Kacip Fatimah (Labisia pumila), an undershrub herb from the Myrsinaceae family, is traditionally used as medicine, particularly by Malay women during post-natal care. The development of L. pumila as a medicinal herb has led to its commercialization and increasing demand. To ensure its sustainable supply, the propagation of kacip Fatimah on a large scale such as through tissue culture needs to be explored. In this study, the leaves of a selected high yielding clone of kacip Fatimah, clone AA28, were used as explants. After surface sterilization, the explants were cultured on MS basal medium containing 0.5 mg/l NAA with different concentrations of BAP ranging from 0.05 to 0.15 mg/l for shoot induction. The explants cultured in MS medium containing 0.5 mg/l NAA in combination with 0.05 mg/l BAP produced the highest mean number of shoots per explants (10 ± 0.1). The shoot multiplication study is in progress and will be reported in a future paper. Penerbit Universiti Kebangsaan Malaysia 2015-04 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/8717/1/44_1_19.pdf Syafiqah Nabilah, S.B. and Farah Fazwa, M.A. and Nor Hasnida, H. and Siti Suhaila, A.R. and Norhayati, S. and Nazirah, A. and Muhammad Fuad, Y. and Haliza, I. and Mohamad, O. (2015) Preliminary study of in vitro propagation of a selected high yielding clone of kacip fatimah (Labisia pumila VAR. alata). Malaysian Applied Biology, 44 (1). pp. 115-118. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=505&catid=59:current-view&Itemid=56
spellingShingle Syafiqah Nabilah, S.B.
Farah Fazwa, M.A.
Nor Hasnida, H.
Siti Suhaila, A.R.
Norhayati, S.
Nazirah, A.
Muhammad Fuad, Y.
Haliza, I.
Mohamad, O.
Preliminary study of in vitro propagation of a selected high yielding clone of kacip fatimah (Labisia pumila VAR. alata)
title Preliminary study of in vitro propagation of a selected high yielding clone of kacip fatimah (Labisia pumila VAR. alata)
title_full Preliminary study of in vitro propagation of a selected high yielding clone of kacip fatimah (Labisia pumila VAR. alata)
title_fullStr Preliminary study of in vitro propagation of a selected high yielding clone of kacip fatimah (Labisia pumila VAR. alata)
title_full_unstemmed Preliminary study of in vitro propagation of a selected high yielding clone of kacip fatimah (Labisia pumila VAR. alata)
title_short Preliminary study of in vitro propagation of a selected high yielding clone of kacip fatimah (Labisia pumila VAR. alata)
title_sort preliminary study of in vitro propagation of a selected high yielding clone of kacip fatimah labisia pumila var alata
url http://journalarticle.ukm.my/8717/1/44_1_19.pdf
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