Quantification of Zonula Occludens-1 mRNA expression in cultu-red bovine retinal endothelial cells, using SYBR green real-time PCR
A simple and rapid real-time reverse transcription-PCR (RT-PCR) technique using SYBR green fluorescence was developed to quantify Zonula Occludens-1 (ZO-1) mRNA expression in histamine-treated cultured bovine retinal microvessel endothelial cells. There was no difference between the quantity of ZO-1...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Biomedical Research Press
2008
|
Subjects: | |
Online Access: | http://eprints.um.edu.my/10273/1/Quantification_of_Zonula_Occludens-1_mRNA_expression_in_cultu-red_bovine_retinal_endothelial_cells%2C_using_SYBR_green_real-time_PCR.pdf |