Quantification of Zonula Occludens-1 mRNA expression in cultu-red bovine retinal endothelial cells, using SYBR green real-time PCR
A simple and rapid real-time reverse transcription-PCR (RT-PCR) technique using SYBR green fluorescence was developed to quantify Zonula Occludens-1 (ZO-1) mRNA expression in histamine-treated cultured bovine retinal microvessel endothelial cells. There was no difference between the quantity of ZO-1...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Biomedical Research Press
2008
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| Online Access: | http://eprints.um.edu.my/10273/1/Quantification_of_Zonula_Occludens-1_mRNA_expression_in_cultu-red_bovine_retinal_endothelial_cells%2C_using_SYBR_green_real-time_PCR.pdf |
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| author | Ismail, I.S. Muniandy, S. Koon, C.Y. Bee, J.C. Rayappan, A.V. Kum, T.W. Qvist, R. |
| author_facet | Ismail, I.S. Muniandy, S. Koon, C.Y. Bee, J.C. Rayappan, A.V. Kum, T.W. Qvist, R. |
| author_sort | Ismail, I.S. |
| collection | UM |
| description | A simple and rapid real-time reverse transcription-PCR (RT-PCR) technique using SYBR green fluorescence was developed to quantify Zonula Occludens-1 (ZO-1) mRNA expression in histamine-treated cultured bovine retinal microvessel endothelial cells. There was no difference between the quantity of ZO-1 mRNA expression in histamine-treated (10-7M to 10-9 M) and untreated bovine retinal endothelial cells at high magnesium levels. Immunohisto-chemistry staining and western blot assay unequivocally demonstrated the approximately similar levels of ZO-1 protein in both histamine-treated and non-treated endothelial cells at high magnesium concentrations. |
| first_indexed | 2024-03-06T05:25:45Z |
| format | Article |
| id | um.eprints-10273 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2024-03-06T05:25:45Z |
| publishDate | 2008 |
| publisher | Biomedical Research Press |
| record_format | dspace |
| spelling | um.eprints-102732014-10-29T06:25:44Z http://eprints.um.edu.my/10273/ Quantification of Zonula Occludens-1 mRNA expression in cultu-red bovine retinal endothelial cells, using SYBR green real-time PCR Ismail, I.S. Muniandy, S. Koon, C.Y. Bee, J.C. Rayappan, A.V. Kum, T.W. Qvist, R. R Medicine (General) A simple and rapid real-time reverse transcription-PCR (RT-PCR) technique using SYBR green fluorescence was developed to quantify Zonula Occludens-1 (ZO-1) mRNA expression in histamine-treated cultured bovine retinal microvessel endothelial cells. There was no difference between the quantity of ZO-1 mRNA expression in histamine-treated (10-7M to 10-9 M) and untreated bovine retinal endothelial cells at high magnesium levels. Immunohisto-chemistry staining and western blot assay unequivocally demonstrated the approximately similar levels of ZO-1 protein in both histamine-treated and non-treated endothelial cells at high magnesium concentrations. Biomedical Research Press 2008 Article PeerReviewed application/pdf en http://eprints.um.edu.my/10273/1/Quantification_of_Zonula_Occludens-1_mRNA_expression_in_cultu-red_bovine_retinal_endothelial_cells%2C_using_SYBR_green_real-time_PCR.pdf Ismail, I.S. and Muniandy, S. and Koon, C.Y. and Bee, J.C. and Rayappan, A.V. and Kum, T.W. and Qvist, R. (2008) Quantification of Zonula Occludens-1 mRNA expression in cultu-red bovine retinal endothelial cells, using SYBR green real-time PCR. Biomedical Research, 19 (2). pp. 117-124. ISSN 0388-6107, |
| spellingShingle | R Medicine (General) Ismail, I.S. Muniandy, S. Koon, C.Y. Bee, J.C. Rayappan, A.V. Kum, T.W. Qvist, R. Quantification of Zonula Occludens-1 mRNA expression in cultu-red bovine retinal endothelial cells, using SYBR green real-time PCR |
| title | Quantification of Zonula Occludens-1 mRNA expression in cultu-red bovine retinal endothelial cells, using SYBR green real-time PCR |
| title_full | Quantification of Zonula Occludens-1 mRNA expression in cultu-red bovine retinal endothelial cells, using SYBR green real-time PCR |
| title_fullStr | Quantification of Zonula Occludens-1 mRNA expression in cultu-red bovine retinal endothelial cells, using SYBR green real-time PCR |
| title_full_unstemmed | Quantification of Zonula Occludens-1 mRNA expression in cultu-red bovine retinal endothelial cells, using SYBR green real-time PCR |
| title_short | Quantification of Zonula Occludens-1 mRNA expression in cultu-red bovine retinal endothelial cells, using SYBR green real-time PCR |
| title_sort | quantification of zonula occludens 1 mrna expression in cultu red bovine retinal endothelial cells using sybr green real time pcr |
| topic | R Medicine (General) |
| url | http://eprints.um.edu.my/10273/1/Quantification_of_Zonula_Occludens-1_mRNA_expression_in_cultu-red_bovine_retinal_endothelial_cells%2C_using_SYBR_green_real-time_PCR.pdf |
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