Stacking gels: A method for maximising output for pulsed-field gel electrophoresis
Pulsed field gel electrophoresis (PFGE), the gold standard of molecular typing methods, has a major disadvantage of an unusually long electrophoretic time. From the original protocol of 6 days, it was modified to 3 days and subsequently to a single day. We describe the procedure of stacking five to...
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Medknow Publications
2009
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