Simulation of improper food hygiene practices: A quantitative assessment of Vibrio parahaemolyticus distribution
Kitchen mishandling practices contribute to a large number of foodborne illnesses. In this study, the transfer and cross-contamination potential of Vibrio parahaemolyticus from bloody clams to ready-to-eat food (lettuce) was assessed. Three scenarios were investigated: 1) direct cross-contamination,...
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| Format: | Article |
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Elsevier
2018
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| _version_ | 1825721672572862464 |
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| author | Malcolm, Tan Turk Hsern Chang, Wei San Loo, Yuet Ying Cheah, Yoke Kqueen Wan Mohamed Radzi, Che Wan Jasimah Kantilal, Haresh Kumar Nishibuchi, Mitsuaki Son, Radu |
| author_facet | Malcolm, Tan Turk Hsern Chang, Wei San Loo, Yuet Ying Cheah, Yoke Kqueen Wan Mohamed Radzi, Che Wan Jasimah Kantilal, Haresh Kumar Nishibuchi, Mitsuaki Son, Radu |
| author_sort | Malcolm, Tan Turk Hsern |
| collection | UM |
| description | Kitchen mishandling practices contribute to a large number of foodborne illnesses. In this study, the transfer and cross-contamination potential of Vibrio parahaemolyticus from bloody clams to ready-to-eat food (lettuce) was assessed. Three scenarios were investigated: 1) direct cross-contamination, the transfer of V. parahaemolyticus from bloody clams to non-food contact surfaces (hands and kitchen utensils) to lettuce (via slicing), was evaluated; 2) perfunctory decontamination, the efficacy of two superficial cleaning treatments: a) rinsing in a pail of water, and b) wiping with a kitchen towel, were determined; and 3) secondary cross-contamination, the microbial transfer from cleaning residuals (wash water or stained kitchen towel) to lettuce was assessed. The mean of percent transfer rates through direct contact was 3.6%, and an average of 3.5% of total V. parahaemolyticus was recovered from sliced lettuce. The attempted treatments reduced the transferred population by 99.0% (rinsing) and 94.5% (wiping), and the relative amount of V. parahaemolyticus on sliced lettuce was reduced to 0.008%. V. parahaemolyticus exposure via secondary cross-contamination was marginal. The relative amount of V. parahaemolyticus recovered from washed lettuce was 0.07%, and the transfers from stained kitchen towel to lettuce were insubstantial. Our study highlights that V. parahaemolyticus was readily spread in the kitchen, potentially through sharing of non-food contact surfaces. Results from this study can be used to better understand and potentially raising the awareness of proper handling practices to avert the spread of foodborne pathogens. |
| first_indexed | 2024-03-06T05:53:55Z |
| format | Article |
| id | um.eprints-21356 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:53:55Z |
| publishDate | 2018 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | um.eprints-213562019-05-28T03:36:33Z http://eprints.um.edu.my/21356/ Simulation of improper food hygiene practices: A quantitative assessment of Vibrio parahaemolyticus distribution Malcolm, Tan Turk Hsern Chang, Wei San Loo, Yuet Ying Cheah, Yoke Kqueen Wan Mohamed Radzi, Che Wan Jasimah Kantilal, Haresh Kumar Nishibuchi, Mitsuaki Son, Radu Q Science (General) T Technology (General) Kitchen mishandling practices contribute to a large number of foodborne illnesses. In this study, the transfer and cross-contamination potential of Vibrio parahaemolyticus from bloody clams to ready-to-eat food (lettuce) was assessed. Three scenarios were investigated: 1) direct cross-contamination, the transfer of V. parahaemolyticus from bloody clams to non-food contact surfaces (hands and kitchen utensils) to lettuce (via slicing), was evaluated; 2) perfunctory decontamination, the efficacy of two superficial cleaning treatments: a) rinsing in a pail of water, and b) wiping with a kitchen towel, were determined; and 3) secondary cross-contamination, the microbial transfer from cleaning residuals (wash water or stained kitchen towel) to lettuce was assessed. The mean of percent transfer rates through direct contact was 3.6%, and an average of 3.5% of total V. parahaemolyticus was recovered from sliced lettuce. The attempted treatments reduced the transferred population by 99.0% (rinsing) and 94.5% (wiping), and the relative amount of V. parahaemolyticus on sliced lettuce was reduced to 0.008%. V. parahaemolyticus exposure via secondary cross-contamination was marginal. The relative amount of V. parahaemolyticus recovered from washed lettuce was 0.07%, and the transfers from stained kitchen towel to lettuce were insubstantial. Our study highlights that V. parahaemolyticus was readily spread in the kitchen, potentially through sharing of non-food contact surfaces. Results from this study can be used to better understand and potentially raising the awareness of proper handling practices to avert the spread of foodborne pathogens. Elsevier 2018 Article PeerReviewed Malcolm, Tan Turk Hsern and Chang, Wei San and Loo, Yuet Ying and Cheah, Yoke Kqueen and Wan Mohamed Radzi, Che Wan Jasimah and Kantilal, Haresh Kumar and Nishibuchi, Mitsuaki and Son, Radu (2018) Simulation of improper food hygiene practices: A quantitative assessment of Vibrio parahaemolyticus distribution. International Journal of Food Microbiology, 284. pp. 112-119. ISSN 0168-1605, DOI https://doi.org/10.1016/j.ijfoodmicro.2018.08.012 <https://doi.org/10.1016/j.ijfoodmicro.2018.08.012>. https://doi.org/10.1016/j.ijfoodmicro.2018.08.012 doi:10.1016/j.ijfoodmicro.2018.08.012 |
| spellingShingle | Q Science (General) T Technology (General) Malcolm, Tan Turk Hsern Chang, Wei San Loo, Yuet Ying Cheah, Yoke Kqueen Wan Mohamed Radzi, Che Wan Jasimah Kantilal, Haresh Kumar Nishibuchi, Mitsuaki Son, Radu Simulation of improper food hygiene practices: A quantitative assessment of Vibrio parahaemolyticus distribution |
| title | Simulation of improper food hygiene practices: A quantitative assessment of Vibrio parahaemolyticus distribution |
| title_full | Simulation of improper food hygiene practices: A quantitative assessment of Vibrio parahaemolyticus distribution |
| title_fullStr | Simulation of improper food hygiene practices: A quantitative assessment of Vibrio parahaemolyticus distribution |
| title_full_unstemmed | Simulation of improper food hygiene practices: A quantitative assessment of Vibrio parahaemolyticus distribution |
| title_short | Simulation of improper food hygiene practices: A quantitative assessment of Vibrio parahaemolyticus distribution |
| title_sort | simulation of improper food hygiene practices a quantitative assessment of vibrio parahaemolyticus distribution |
| topic | Q Science (General) T Technology (General) |
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