Conformational destabilization of Bacillus licheniformis α-amylase induced by lysine modification and calcium depletion
Bacillus licheniformis α-amylase (BLA) was chemically modified using 100-fold molar excess of succinic anhydride over protein or 0.66 M potassium cyanate to obtain 42 % succinylated and 81 % carbamylated BLAs. Size and charge homogeneity of modified preparations was established by Sephacryl S-200 HR...
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| Format: | Article |
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Polskie Towarzystwo Biochemiczne
2011
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