Conformational destabilization of Bacillus licheniformis α-amylase induced by lysine modification and calcium depletion

Conformational destabilization of Bacillus licheniformis α-amylase induced by lysine modification and calcium depletion

Bacillus licheniformis α-amylase (BLA) was chemically modified using 100-fold molar excess of succinic anhydride over protein or 0.66 M potassium cyanate to obtain 42 % succinylated and 81 % carbamylated BLAs. Size and charge homogeneity of modified preparations was established by Sephacryl S-200 HR...

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Bibliographic Details
Main Authors:	Tan, C.Y., Rahman, R.N., Kadir, H.A., Tayyab, S.
Format:	Article
Published:	Polskie Towarzystwo Biochemiczne 2011
Subjects:	R Medicine

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