Inadvertent expression of dengue 2 virus NS3-EGFP fusion protein in Escherichia coli using the pEGFP-N1 mammalian expression vector

Dengue 2 New Guinea C (NGC) virus NS3 protein, a potentially important virulence factor was cloned to the N-terminus of the Aequoreo t.rictorio enhanced green fluorescent protein (EGFP) using the pEGFP-N1 manimaiian expression vector. During amplification of the recombinant plasmid in E. coli, trans...

Full description

Bibliographic Details
Main Authors: Shafee, Norazizah, AbuBakar, Sazaly
Format: Article
Published: Faculty of Medicine, University of Malaya 1999
Subjects: