Characterization of secreted recombinant Toxoplasma gondii surface antigen 2 (SAG2) heterologously expressed by the yeast Pichia pastoris

The surface antigen 2 (SAG2) gene of the protozoan parasite, Toxoplasma gondii, was cloned and extracellularly expressed in the yeast Pichia pastoris. The effectiveness of the secreted recombinant SAG2 (rSAG2-S) as a serodiagnosis reagent was assessed by western blots and ELISA. In the western blot...

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Bibliographic Details
Main Authors: Fong, M.Y., Lau, Y.L., Zulqarnain, M.
Format: Article
Language:English
Published: Kluwer (now part of Springer) 2008
Subjects:
Online Access:http://eprints.um.edu.my/2723/1/Characterization_of_secreted_recombinant_Toxoplasma_gondii_surface_antigen_2_%28SAG2%29_heterologously_expressed_by_the_yeast_Pichia_pastoris.pdf