Direct cloning approach for expression of an anti-cucumber mosaic virus single-chain variable fragment in plant

An anti Cucumber Mosaic Virus (anti-CMV) single chain variable fragment (scFv) was constructed into a plant expression vector using self-designed primers. Initially, sequence information of the scFv was obtained through automated sequencing. Based on the sequence information, two primers with approp...

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Bibliographic Details
Main Authors: Chua, K.H., Khalid, N., Harikrishna, J.A., Othman, R.Y.
Format: Article
Language:English
Published: 2005
Subjects:
Online Access:http://eprints.um.edu.my/3806/1/Direct_cloning_approach_for_expression_of_an_anti-cucumber_mosaic_virus_single-chain_variable_fragment_in_plant..pdf