Gender identification of mouse embryos using the polymerase chain reaction (PCR) technique
A multiplex nested PCR technique was used to identify gender from single blastomeres biopsied from 8-cell mouse embryos. The primers amplified sequences of the Sry and Zfy genes on the Y-chromosome, and a polymorphic X chromosome microsatellite locus (DXNds-3). Amplification of male DNA gave three b...
| Main Authors: | , , , , , |
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| Format: | Article |
| Published: |
2006
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| Subjects: |