Zinc (II) complex with a cationic Schiff base ligand: Synthesis, characterization, and biological studies
A cationic Schiff base ligand, TSB (L) and its Zn (II) complex (1) were synthesized and characterized by using CHN, 1H-NMR, FT-IR, UV, LC-MS, and X-ray methods. Their ability to inhibit topoisomerase I, DNA cleavage activities, and cytotoxicity were studied. X-ray diffraction study shows that the mo...
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| Format: | Article |
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2013
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| author | Lee, S.K. Tan, K.W. Ng, S.W. Ooi, K.K. Ang, K.P. Abdah, M.A. |
| author_facet | Lee, S.K. Tan, K.W. Ng, S.W. Ooi, K.K. Ang, K.P. Abdah, M.A. |
| author_sort | Lee, S.K. |
| collection | UM |
| description | A cationic Schiff base ligand, TSB (L) and its Zn (II) complex (1) were synthesized and characterized by using CHN, 1H-NMR, FT-IR, UV, LC-MS, and X-ray methods. Their ability to inhibit topoisomerase I, DNA cleavage activities, and cytotoxicity were studied. X-ray diffraction study shows that the mononuclear complex 1 is four coordinated with distorted tetrahedral geometry. The singly deprotonated Schiff base ligand L acts as a bidentate ON-donor ligand. Complexation of L increases the inhibitory strength on topoisomerase I activity. Complex 1 could fully inhibit topoisomerase I activity at 250μM, while L did not show any inhibitory effect on topoisomerase I activity. In addition, L and complex 1 could cleave pBR322 DNA in a concentration and time dependent profile. Surprisingly, L has better DNA cleavage activity than complex 1. The cleavage of DNA by complex 1 is altered in the presence of hydrogen peroxide. Furthermore, L and complex 1 are mildly cytotoxic towards human ovarian cancer A2780 and hepatocellular carcinoma HepG2. |
| first_indexed | 2024-03-06T05:21:30Z |
| format | Article |
| id | um.eprints-8562 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:21:30Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | um.eprints-85622013-11-21T09:26:55Z http://eprints.um.edu.my/8562/ Zinc (II) complex with a cationic Schiff base ligand: Synthesis, characterization, and biological studies Lee, S.K. Tan, K.W. Ng, S.W. Ooi, K.K. Ang, K.P. Abdah, M.A. Q Science (General) A cationic Schiff base ligand, TSB (L) and its Zn (II) complex (1) were synthesized and characterized by using CHN, 1H-NMR, FT-IR, UV, LC-MS, and X-ray methods. Their ability to inhibit topoisomerase I, DNA cleavage activities, and cytotoxicity were studied. X-ray diffraction study shows that the mononuclear complex 1 is four coordinated with distorted tetrahedral geometry. The singly deprotonated Schiff base ligand L acts as a bidentate ON-donor ligand. Complexation of L increases the inhibitory strength on topoisomerase I activity. Complex 1 could fully inhibit topoisomerase I activity at 250μM, while L did not show any inhibitory effect on topoisomerase I activity. In addition, L and complex 1 could cleave pBR322 DNA in a concentration and time dependent profile. Surprisingly, L has better DNA cleavage activity than complex 1. The cleavage of DNA by complex 1 is altered in the presence of hydrogen peroxide. Furthermore, L and complex 1 are mildly cytotoxic towards human ovarian cancer A2780 and hepatocellular carcinoma HepG2. 2013 Article PeerReviewed Lee, S.K. and Tan, K.W. and Ng, S.W. and Ooi, K.K. and Ang, K.P. and Abdah, M.A. (2013) Zinc (II) complex with a cationic Schiff base ligand: Synthesis, characterization, and biological studies. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. ISSN 1386-1425, DOI PMID: 24231745. http://www.ncbi.nlm.nih.gov/pubmed/24231745 PMID: 24231745 |
| spellingShingle | Q Science (General) Lee, S.K. Tan, K.W. Ng, S.W. Ooi, K.K. Ang, K.P. Abdah, M.A. Zinc (II) complex with a cationic Schiff base ligand: Synthesis, characterization, and biological studies |
| title | Zinc (II) complex with a cationic Schiff base ligand: Synthesis, characterization, and biological studies |
| title_full | Zinc (II) complex with a cationic Schiff base ligand: Synthesis, characterization, and biological studies |
| title_fullStr | Zinc (II) complex with a cationic Schiff base ligand: Synthesis, characterization, and biological studies |
| title_full_unstemmed | Zinc (II) complex with a cationic Schiff base ligand: Synthesis, characterization, and biological studies |
| title_short | Zinc (II) complex with a cationic Schiff base ligand: Synthesis, characterization, and biological studies |
| title_sort | zinc ii complex with a cationic schiff base ligand synthesis characterization and biological studies |
| topic | Q Science (General) |
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