Phytochemicals screening for potential antibacterial and antioxidant properties in Sabah pigmented rice

The phytochemical contents, antibacterial, antioxidant and free radical scavenging properties of four pigmented rice varieties (black, red, brown and white) in Sa bah were studied. Seven phytochemical groups, which are the alkaloids, phenolic acids, saponins, flavonoids, tocopherols, carbohydrate a...

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Main Author: Chong, Pui Lai
Format: Academic Exercise
Language:English
Published: 2011
Subjects:
Online Access:https://eprints.ums.edu.my/id/eprint/17390/1/Phytochemicals_screening_for_potential_antibacterial.pdf
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author Chong, Pui Lai
author_facet Chong, Pui Lai
author_sort Chong, Pui Lai
collection UMS
description The phytochemical contents, antibacterial, antioxidant and free radical scavenging properties of four pigmented rice varieties (black, red, brown and white) in Sa bah were studied. Seven phytochemical groups, which are the alkaloids, phenolic acids, saponins, flavonoids, tocopherols, carbohydrate and proteins were found to be present in all the rice samples. Red rice contains the highest quantity of phenolic acids (329.93 ± 19.17 mg/100 g), saponins (625.33 ± 10.26 mg/100 g), tocopherols (200.33 ± 13.61 mg/100 g), and protein (22.45 ± 0.98%). Black rice has the highest content of flavonoids (23.28 ±0.97 mg/100 g). There are no significant differences in the content of alkaloids and carbohydrate among all four rice varieties. In order to detect the antioxidant activity of the pigmented rice extracts, ferric thiocyanate (FTC) assay was used and compared with the thiobarbituric acid (TBA) method. Scavenging activity was measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals. Red rice extract shows the highest activity in all three tests, FTC (0.302), TBA (0.329) and DPPH (65.54% ± 0.57) due to its high content of phenolic acids and tocopherols, followed in descending order by black rice, brown rice and finally white rice. Potential antibacterial activity of the methanolic crude extracts of pigmented rice was screened against Escherichia coli, Pseudomonas sp. and Streptococcus sp. using disc diffusion technique. There are no inhibition zone of bacteria was formed that indicated all the four rice varieties do not have antibacterial properties. This study indicates that pigmented rice varieties has protective effect against hydroperoxide generation and has free radical scavenging activity. The antioxidants capacity of the extract also differs depending on the bran colour of the rice.
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spelling ums.eprints-173902017-10-16T04:18:44Z https://eprints.ums.edu.my/id/eprint/17390/ Phytochemicals screening for potential antibacterial and antioxidant properties in Sabah pigmented rice Chong, Pui Lai SB Plant culture The phytochemical contents, antibacterial, antioxidant and free radical scavenging properties of four pigmented rice varieties (black, red, brown and white) in Sa bah were studied. Seven phytochemical groups, which are the alkaloids, phenolic acids, saponins, flavonoids, tocopherols, carbohydrate and proteins were found to be present in all the rice samples. Red rice contains the highest quantity of phenolic acids (329.93 ± 19.17 mg/100 g), saponins (625.33 ± 10.26 mg/100 g), tocopherols (200.33 ± 13.61 mg/100 g), and protein (22.45 ± 0.98%). Black rice has the highest content of flavonoids (23.28 ±0.97 mg/100 g). There are no significant differences in the content of alkaloids and carbohydrate among all four rice varieties. In order to detect the antioxidant activity of the pigmented rice extracts, ferric thiocyanate (FTC) assay was used and compared with the thiobarbituric acid (TBA) method. Scavenging activity was measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals. Red rice extract shows the highest activity in all three tests, FTC (0.302), TBA (0.329) and DPPH (65.54% ± 0.57) due to its high content of phenolic acids and tocopherols, followed in descending order by black rice, brown rice and finally white rice. Potential antibacterial activity of the methanolic crude extracts of pigmented rice was screened against Escherichia coli, Pseudomonas sp. and Streptococcus sp. using disc diffusion technique. There are no inhibition zone of bacteria was formed that indicated all the four rice varieties do not have antibacterial properties. This study indicates that pigmented rice varieties has protective effect against hydroperoxide generation and has free radical scavenging activity. The antioxidants capacity of the extract also differs depending on the bran colour of the rice. 2011 Academic Exercise NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/17390/1/Phytochemicals_screening_for_potential_antibacterial.pdf Chong, Pui Lai (2011) Phytochemicals screening for potential antibacterial and antioxidant properties in Sabah pigmented rice. Universiti Malaysia Sabah. (Unpublished)
spellingShingle SB Plant culture
Chong, Pui Lai
Phytochemicals screening for potential antibacterial and antioxidant properties in Sabah pigmented rice
title Phytochemicals screening for potential antibacterial and antioxidant properties in Sabah pigmented rice
title_full Phytochemicals screening for potential antibacterial and antioxidant properties in Sabah pigmented rice
title_fullStr Phytochemicals screening for potential antibacterial and antioxidant properties in Sabah pigmented rice
title_full_unstemmed Phytochemicals screening for potential antibacterial and antioxidant properties in Sabah pigmented rice
title_short Phytochemicals screening for potential antibacterial and antioxidant properties in Sabah pigmented rice
title_sort phytochemicals screening for potential antibacterial and antioxidant properties in sabah pigmented rice
topic SB Plant culture
url https://eprints.ums.edu.my/id/eprint/17390/1/Phytochemicals_screening_for_potential_antibacterial.pdf
work_keys_str_mv AT chongpuilai phytochemicalsscreeningforpotentialantibacterialandantioxidantpropertiesinsabahpigmentedrice