Effectiveness of DNase and washing steps in removing dead cells' DNA for PCR detection of viable Escherichia coli
This study investigates the use of DNase and washing steps in removing dead cells’ DNA during sample preparation and their effect on the detection of viable cells using polymerase chain reaction (PCR). The results indicated that the DNA from heat-killed cells could be completely removed by DNase; th...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English English |
| Published: |
69(6) · December 2012
2012
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| Online Access: | https://eprints.ums.edu.my/id/eprint/21616/1/Effectiveness%20of%20DNase%20and%20washing%20steps%20in%20removing%20dead%20cells.pdf https://eprints.ums.edu.my/id/eprint/21616/7/Effectiveness%20of%20DNase%20and%20washing%20steps%20in%20removing%20dead%20cells%27%20DNA%20for%20PCR%20detection%20of%20viable%20Escherichia%20coli.pdf |
Internet
https://eprints.ums.edu.my/id/eprint/21616/1/Effectiveness%20of%20DNase%20and%20washing%20steps%20in%20removing%20dead%20cells.pdfhttps://eprints.ums.edu.my/id/eprint/21616/7/Effectiveness%20of%20DNase%20and%20washing%20steps%20in%20removing%20dead%20cells%27%20DNA%20for%20PCR%20detection%20of%20viable%20Escherichia%20coli.pdf