Determination of Chemical Composition, Total Flavonoid Content, Total Phenolic Content and Antioxidant Capacity of Various Crude Extracts of Manihot esculenta Crantz Leaves
In the present study, the leaves of Manihot esculenta Crantz were extracted by various organic solvents viz. hexane, chloroform, ethyl acetate and subjected to GC-MS analysis to determine their chemical composition and antioxidant activity by DPPH assay. The GC-MS analysis of hexane extract displaye...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
2018
|
| Subjects: | |
| Online Access: | https://eprints.ums.edu.my/id/eprint/22901/1/Determination%20of%20Chemical%20Composition%2C%20Total%20Flavonoid%20Content%2C%20Total%20Phenolic%20Content%20and%20Antioxidant%20Capacity%20of%20Various%20Crude%20Extracts%20of%20Manihot%20esculenta%20Crantz%20Leaves.pdf |
| _version_ | 1825713674775429120 |
|---|---|
| author | Mohammad Shaheen Khan Samina Khan Yusufzai Lawrance Kimin Noor Adzianni Nabila Binti Jabi |
| author_facet | Mohammad Shaheen Khan Samina Khan Yusufzai Lawrance Kimin Noor Adzianni Nabila Binti Jabi |
| author_sort | Mohammad Shaheen Khan |
| collection | UMS |
| description | In the present study, the leaves of Manihot esculenta Crantz were extracted by various organic solvents viz. hexane, chloroform, ethyl acetate and subjected to GC-MS analysis to determine their chemical composition and antioxidant activity by DPPH assay. The GC-MS analysis of hexane extract displayed squalene (10.25%), 9-octadecenoic acid (Z)-, methyl ester (9.01%), phytol (8.71%) and hexadecanoic acid, methyl ester (8.33%) as major compounds. The major compounds present in the chloroform extract were β-amyrin (12.70%), phytol (11.35%), 1,4-methanoazulen-9-ol, decahydro-1,5,5,8atetramethyl-,[1R- (1α,3aβ,4α,8aβ,9S*)]- (10.98%), 6β-Bicyclo[4.3.0]nonane, 5β-iodomethyl-1β-isopropenyl-4α,5α-dimethyl-, (9.40%) and humulane-1,6-dien-3-ol (8.53%). In ethyl acetate extract, squalene (16.39%), 9,11-dimethyltetracyclo [7.3.1.0(2.7).1(7.11)]tetradecane (12.01%) and humulane-1,6-dien-3-ol (9.58%) were reported as major compounds. The total phenolic content for hexane, chloroform and ethyl acetate was 5.35 ± 0.66 mg GAE/g, 7.52 ± 0.09 mg GAE/g and 13.47 ± 0.56 mg GAE/g, respectively. Whereas the total flavonoid content for hexane, chloroform and ethyl acetate was 0.89 ± 0.17 mg QE/g, 2.69 ± 0.09 mg QE/g and 6.66 ± 0.94 mg QE/g, respectively. The antioxidant activity of ethyl acetate extract (IC50 = 0.19 mg/mL) was higher than chloroform (IC50 = 1.39 mg/mL) and hexane extracts (IC50 = 1.74 mg/mL), with respect to the standard butylatedhydroxytoluene (BHT) (IC50 = 0.04 mg/mL). |
| first_indexed | 2024-03-06T03:00:13Z |
| format | Article |
| id | ums.eprints-22901 |
| institution | Universiti Malaysia Sabah |
| language | English |
| last_indexed | 2024-03-06T03:00:13Z |
| publishDate | 2018 |
| record_format | dspace |
| spelling | ums.eprints-229012019-07-22T04:20:41Z https://eprints.ums.edu.my/id/eprint/22901/ Determination of Chemical Composition, Total Flavonoid Content, Total Phenolic Content and Antioxidant Capacity of Various Crude Extracts of Manihot esculenta Crantz Leaves Mohammad Shaheen Khan Samina Khan Yusufzai Lawrance Kimin Noor Adzianni Nabila Binti Jabi QK Botany In the present study, the leaves of Manihot esculenta Crantz were extracted by various organic solvents viz. hexane, chloroform, ethyl acetate and subjected to GC-MS analysis to determine their chemical composition and antioxidant activity by DPPH assay. The GC-MS analysis of hexane extract displayed squalene (10.25%), 9-octadecenoic acid (Z)-, methyl ester (9.01%), phytol (8.71%) and hexadecanoic acid, methyl ester (8.33%) as major compounds. The major compounds present in the chloroform extract were β-amyrin (12.70%), phytol (11.35%), 1,4-methanoazulen-9-ol, decahydro-1,5,5,8atetramethyl-,[1R- (1α,3aβ,4α,8aβ,9S*)]- (10.98%), 6β-Bicyclo[4.3.0]nonane, 5β-iodomethyl-1β-isopropenyl-4α,5α-dimethyl-, (9.40%) and humulane-1,6-dien-3-ol (8.53%). In ethyl acetate extract, squalene (16.39%), 9,11-dimethyltetracyclo [7.3.1.0(2.7).1(7.11)]tetradecane (12.01%) and humulane-1,6-dien-3-ol (9.58%) were reported as major compounds. The total phenolic content for hexane, chloroform and ethyl acetate was 5.35 ± 0.66 mg GAE/g, 7.52 ± 0.09 mg GAE/g and 13.47 ± 0.56 mg GAE/g, respectively. Whereas the total flavonoid content for hexane, chloroform and ethyl acetate was 0.89 ± 0.17 mg QE/g, 2.69 ± 0.09 mg QE/g and 6.66 ± 0.94 mg QE/g, respectively. The antioxidant activity of ethyl acetate extract (IC50 = 0.19 mg/mL) was higher than chloroform (IC50 = 1.39 mg/mL) and hexane extracts (IC50 = 1.74 mg/mL), with respect to the standard butylatedhydroxytoluene (BHT) (IC50 = 0.04 mg/mL). 2018-04 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/22901/1/Determination%20of%20Chemical%20Composition%2C%20Total%20Flavonoid%20Content%2C%20Total%20Phenolic%20Content%20and%20Antioxidant%20Capacity%20of%20Various%20Crude%20Extracts%20of%20Manihot%20esculenta%20Crantz%20Leaves.pdf Mohammad Shaheen Khan and Samina Khan Yusufzai and Lawrance Kimin and Noor Adzianni Nabila Binti Jabi (2018) Determination of Chemical Composition, Total Flavonoid Content, Total Phenolic Content and Antioxidant Capacity of Various Crude Extracts of Manihot esculenta Crantz Leaves. International Journal for Research in Applied Science & Engineering Technology, 6 (4). pp. 2433-2443. ISSN 2321-9653 |
| spellingShingle | QK Botany Mohammad Shaheen Khan Samina Khan Yusufzai Lawrance Kimin Noor Adzianni Nabila Binti Jabi Determination of Chemical Composition, Total Flavonoid Content, Total Phenolic Content and Antioxidant Capacity of Various Crude Extracts of Manihot esculenta Crantz Leaves |
| title | Determination of Chemical Composition, Total Flavonoid Content, Total Phenolic Content and Antioxidant Capacity of Various Crude Extracts of Manihot esculenta Crantz Leaves |
| title_full | Determination of Chemical Composition, Total Flavonoid Content, Total Phenolic Content and Antioxidant Capacity of Various Crude Extracts of Manihot esculenta Crantz Leaves |
| title_fullStr | Determination of Chemical Composition, Total Flavonoid Content, Total Phenolic Content and Antioxidant Capacity of Various Crude Extracts of Manihot esculenta Crantz Leaves |
| title_full_unstemmed | Determination of Chemical Composition, Total Flavonoid Content, Total Phenolic Content and Antioxidant Capacity of Various Crude Extracts of Manihot esculenta Crantz Leaves |
| title_short | Determination of Chemical Composition, Total Flavonoid Content, Total Phenolic Content and Antioxidant Capacity of Various Crude Extracts of Manihot esculenta Crantz Leaves |
| title_sort | determination of chemical composition total flavonoid content total phenolic content and antioxidant capacity of various crude extracts of manihot esculenta crantz leaves |
| topic | QK Botany |
| url | https://eprints.ums.edu.my/id/eprint/22901/1/Determination%20of%20Chemical%20Composition%2C%20Total%20Flavonoid%20Content%2C%20Total%20Phenolic%20Content%20and%20Antioxidant%20Capacity%20of%20Various%20Crude%20Extracts%20of%20Manihot%20esculenta%20Crantz%20Leaves.pdf |
| work_keys_str_mv | AT mohammadshaheenkhan determinationofchemicalcompositiontotalflavonoidcontenttotalphenoliccontentandantioxidantcapacityofvariouscrudeextractsofmanihotesculentacrantzleaves AT saminakhanyusufzai determinationofchemicalcompositiontotalflavonoidcontenttotalphenoliccontentandantioxidantcapacityofvariouscrudeextractsofmanihotesculentacrantzleaves AT lawrancekimin determinationofchemicalcompositiontotalflavonoidcontenttotalphenoliccontentandantioxidantcapacityofvariouscrudeextractsofmanihotesculentacrantzleaves AT nooradzianninabilabintijabi determinationofchemicalcompositiontotalflavonoidcontenttotalphenoliccontentandantioxidantcapacityofvariouscrudeextractsofmanihotesculentacrantzleaves |