Optimization of an innovative hydrothermal processing on prebiotic properties of eucheuma denticulatum, a tropical red seaweed
Seaweed is a sustainable source of marine oligosaccharides potentially could be used as a prebiotic ingredient for the functional food development. The study aims to optimize the oligosaccharide preparation through thermal hydrolysis of an under-utilized red seaweed, Eucheuma denticulatum. Response...
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| Format: | Conference or Workshop Item |
| Language: | English English |
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2022
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| Online Access: | https://eprints.ums.edu.my/id/eprint/35268/2/FULL%20TEXT.pdf https://eprints.ums.edu.my/id/eprint/35268/5/ABSTRACT.pdf |
| _version_ | 1796911726412693504 |
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| author | Birdie Scott Padam Chee Kiong Siew Fook Yee Chye |
| author_facet | Birdie Scott Padam Chee Kiong Siew Fook Yee Chye |
| author_sort | Birdie Scott Padam |
| collection | UMS |
| description | Seaweed is a sustainable source of marine oligosaccharides potentially could be used as a prebiotic ingredient for the functional food development. The study aims to optimize the oligosaccharide preparation through thermal hydrolysis of an under-utilized red seaweed, Eucheuma denticulatum. Response Surface Methodology (RSM) applying the Box-Behnken Design (BBD) was used on three parameters including temperature (105ºC-135ºC), hydrolysis time (15-35 min) and sulfuric acid concentration (0.05-0.2M). Optimized fractions with good prebiotic activity were characterized using HPLC-SEC and FT-IR. Oligosaccharides fraction ED-F1 was shown to promote the growth of beneficial gut microbiota including Lactobacillus plantarum, L. casei, L. acidophilus, Bifidobacterium animalis and B. longum with the highest prebiotic activity score of 1.64±0.17. The optimization studies showed that hydrolysis time was the most significant parameter for the oligosaccharides yield. Optimal hydrolysis conditions for ED-F1 were 120ºC, 21 min, 0.12M H2SO4 with the highest yield achieved (11.15 g/100 g of dry weight). Molecular weight of ED-F1 was determined at 1025 Da while FT-IR analysis revealed the presence of sulfated oligosaccharides with similar characteristics of i-carrageenan. These findings signify the innovative method for the efficient production of seaweed derived prebiotic oligosaccharides, which could be a promising source of functional food ingredients for the development of health foods and beverages. |
| first_indexed | 2024-03-06T03:22:57Z |
| format | Conference or Workshop Item |
| id | ums.eprints-35268 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T03:22:57Z |
| publishDate | 2022 |
| record_format | dspace |
| spelling | ums.eprints-352682023-05-22T02:51:34Z https://eprints.ums.edu.my/id/eprint/35268/ Optimization of an innovative hydrothermal processing on prebiotic properties of eucheuma denticulatum, a tropical red seaweed Birdie Scott Padam Chee Kiong Siew Fook Yee Chye HD9000-9495 Agricultural industries QR75-99.5 Bacteria Seaweed is a sustainable source of marine oligosaccharides potentially could be used as a prebiotic ingredient for the functional food development. The study aims to optimize the oligosaccharide preparation through thermal hydrolysis of an under-utilized red seaweed, Eucheuma denticulatum. Response Surface Methodology (RSM) applying the Box-Behnken Design (BBD) was used on three parameters including temperature (105ºC-135ºC), hydrolysis time (15-35 min) and sulfuric acid concentration (0.05-0.2M). Optimized fractions with good prebiotic activity were characterized using HPLC-SEC and FT-IR. Oligosaccharides fraction ED-F1 was shown to promote the growth of beneficial gut microbiota including Lactobacillus plantarum, L. casei, L. acidophilus, Bifidobacterium animalis and B. longum with the highest prebiotic activity score of 1.64±0.17. The optimization studies showed that hydrolysis time was the most significant parameter for the oligosaccharides yield. Optimal hydrolysis conditions for ED-F1 were 120ºC, 21 min, 0.12M H2SO4 with the highest yield achieved (11.15 g/100 g of dry weight). Molecular weight of ED-F1 was determined at 1025 Da while FT-IR analysis revealed the presence of sulfated oligosaccharides with similar characteristics of i-carrageenan. These findings signify the innovative method for the efficient production of seaweed derived prebiotic oligosaccharides, which could be a promising source of functional food ingredients for the development of health foods and beverages. 2022 Conference or Workshop Item PeerReviewed text en https://eprints.ums.edu.my/id/eprint/35268/2/FULL%20TEXT.pdf text en https://eprints.ums.edu.my/id/eprint/35268/5/ABSTRACT.pdf Birdie Scott Padam and Chee Kiong Siew and Fook Yee Chye (2022) Optimization of an innovative hydrothermal processing on prebiotic properties of eucheuma denticulatum, a tropical red seaweed. In: INTERNATIONAL CONFERENCE ON FOOD SCIENCE AND NUTRITION 2022 ” Future Food: Emerging Trends, Health and Diversity”, 24th-25th August 2022., Online. https://www.mdpi.com/2076-3417/13/3/1517 |
| spellingShingle | HD9000-9495 Agricultural industries QR75-99.5 Bacteria Birdie Scott Padam Chee Kiong Siew Fook Yee Chye Optimization of an innovative hydrothermal processing on prebiotic properties of eucheuma denticulatum, a tropical red seaweed |
| title | Optimization of an innovative hydrothermal processing on prebiotic properties of eucheuma denticulatum, a tropical red seaweed |
| title_full | Optimization of an innovative hydrothermal processing on prebiotic properties of eucheuma denticulatum, a tropical red seaweed |
| title_fullStr | Optimization of an innovative hydrothermal processing on prebiotic properties of eucheuma denticulatum, a tropical red seaweed |
| title_full_unstemmed | Optimization of an innovative hydrothermal processing on prebiotic properties of eucheuma denticulatum, a tropical red seaweed |
| title_short | Optimization of an innovative hydrothermal processing on prebiotic properties of eucheuma denticulatum, a tropical red seaweed |
| title_sort | optimization of an innovative hydrothermal processing on prebiotic properties of eucheuma denticulatum a tropical red seaweed |
| topic | HD9000-9495 Agricultural industries QR75-99.5 Bacteria |
| url | https://eprints.ums.edu.my/id/eprint/35268/2/FULL%20TEXT.pdf https://eprints.ums.edu.my/id/eprint/35268/5/ABSTRACT.pdf |
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