2-dodecanol (decyl methyl carbinol) inhibits hyphal formation and SIR2 expression in C. albicans
Candida albicans is capable of undergoing yeast-hypha transition to attain pathogenicity in humans. In this study, we investigated the differential expression of CaSIR2 via quantitative real-time PCR (qPCR), during yeast-hypha transition with and without the presence of 2-dodecanol. SIR2 transcript...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley-VCH Verlag GmbH & Co. KGaA
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/12634/1/2.pdf |
| _version_ | 1796968268072747008 |
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| author | Lim, Crystale Siew Ying Wong, Won Fen Rosli, Rozita Ng, Kee Peng Seow, Heng Fong Chong, Pei Pei |
| author_facet | Lim, Crystale Siew Ying Wong, Won Fen Rosli, Rozita Ng, Kee Peng Seow, Heng Fong Chong, Pei Pei |
| author_sort | Lim, Crystale Siew Ying |
| collection | UPM |
| description | Candida albicans is capable of undergoing yeast-hypha transition to attain pathogenicity in humans. In this study, we investigated the differential expression of CaSIR2 via quantitative real-time PCR (qPCR), during yeast-hypha transition with and without the presence of 2-dodecanol. SIR2 transcript levels were found to be significantly enhanced after hyphal induction as compared to the yeast form. This study found that 2-dodecanol is able to inhibit hyphal development and block SIR2 up-regulation, even in hyphal-inducing growth conditions. We suggest that SIR2 may be involved in Candida albicans quorum-sensing and serum-induced yeast-hyphae transition via the Ras1-cAMP-Efg1 signalling cascade. |
| first_indexed | 2024-03-06T07:25:57Z |
| format | Article |
| id | upm.eprints-12634 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:25:57Z |
| publishDate | 2009 |
| publisher | Wiley-VCH Verlag GmbH & Co. KGaA |
| record_format | dspace |
| spelling | upm.eprints-126342017-03-28T13:51:46Z http://psasir.upm.edu.my/id/eprint/12634/ 2-dodecanol (decyl methyl carbinol) inhibits hyphal formation and SIR2 expression in C. albicans Lim, Crystale Siew Ying Wong, Won Fen Rosli, Rozita Ng, Kee Peng Seow, Heng Fong Chong, Pei Pei Candida albicans is capable of undergoing yeast-hypha transition to attain pathogenicity in humans. In this study, we investigated the differential expression of CaSIR2 via quantitative real-time PCR (qPCR), during yeast-hypha transition with and without the presence of 2-dodecanol. SIR2 transcript levels were found to be significantly enhanced after hyphal induction as compared to the yeast form. This study found that 2-dodecanol is able to inhibit hyphal development and block SIR2 up-regulation, even in hyphal-inducing growth conditions. We suggest that SIR2 may be involved in Candida albicans quorum-sensing and serum-induced yeast-hyphae transition via the Ras1-cAMP-Efg1 signalling cascade. Wiley-VCH Verlag GmbH & Co. KGaA 2009-11 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/12634/1/2.pdf Lim, Crystale Siew Ying and Wong, Won Fen and Rosli, Rozita and Ng, Kee Peng and Seow, Heng Fong and Chong, Pei Pei (2009) 2-dodecanol (decyl methyl carbinol) inhibits hyphal formation and SIR2 expression in C. albicans. Journal of Basic Microbiology, 49 (6). pp. 579-583. ISSN 0233-111X; ESSN: 1521-4028 10.1002/jobm.200900035 |
| spellingShingle | Lim, Crystale Siew Ying Wong, Won Fen Rosli, Rozita Ng, Kee Peng Seow, Heng Fong Chong, Pei Pei 2-dodecanol (decyl methyl carbinol) inhibits hyphal formation and SIR2 expression in C. albicans |
| title | 2-dodecanol (decyl methyl carbinol) inhibits hyphal formation and SIR2 expression in C. albicans |
| title_full | 2-dodecanol (decyl methyl carbinol) inhibits hyphal formation and SIR2 expression in C. albicans |
| title_fullStr | 2-dodecanol (decyl methyl carbinol) inhibits hyphal formation and SIR2 expression in C. albicans |
| title_full_unstemmed | 2-dodecanol (decyl methyl carbinol) inhibits hyphal formation and SIR2 expression in C. albicans |
| title_short | 2-dodecanol (decyl methyl carbinol) inhibits hyphal formation and SIR2 expression in C. albicans |
| title_sort | 2 dodecanol decyl methyl carbinol inhibits hyphal formation and sir2 expression in c albicans |
| url | http://psasir.upm.edu.my/id/eprint/12634/1/2.pdf |
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