Calcium-induced stabilization of α-amylase against guanidine hydrochloride denaturation
Guanidine hydrochloride (GdnHCl) denaturation of native and Ca-depleted Bacillus licheniformis α-amylase (BLA) was investigated both in the absence and presence of 2 mM calcium chloride (CaCl2)using circular dichroism, fluorescence spectroscopy and biological activity. In both states (Cadepleted and...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Academic Journals
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/13474/1/13474.pdf |