Calcium-induced stabilization of α-amylase against guanidine hydrochloride denaturation
Guanidine hydrochloride (GdnHCl) denaturation of native and Ca-depleted Bacillus licheniformis α-amylase (BLA) was investigated both in the absence and presence of 2 mM calcium chloride (CaCl2)using circular dichroism, fluorescence spectroscopy and biological activity. In both states (Cadepleted and...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Academic Journals
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/13474/1/13474.pdf |
| _version_ | 1796968444526067712 |
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| author | Tan, Cheau Yuaan Raja Abdul Rahman, Raja Noor Zaliha Abdul Kadir, Habsah Tayya, Saad |
| author_facet | Tan, Cheau Yuaan Raja Abdul Rahman, Raja Noor Zaliha Abdul Kadir, Habsah Tayya, Saad |
| author_sort | Tan, Cheau Yuaan |
| collection | UPM |
| description | Guanidine hydrochloride (GdnHCl) denaturation of native and Ca-depleted Bacillus licheniformis α-amylase (BLA) was investigated both in the absence and presence of 2 mM calcium chloride (CaCl2)using circular dichroism, fluorescence spectroscopy and biological activity. In both states (Cadepleted and native form), the protein was denatured to a considerable extent in the absence of 2 mM
CaCl2 with concomitant loss of biological activity upon increasing GdnHCl concentration. On the other hand, this effect was significantly reduced when 2 mM CaCl2 was included in the incubation mixture as revealed by a higher relative mean residue ellipticity, higher relative fluorescence intensity, smaller change in emission maximum and lesser reduction in biological activity. Interestingly, using these probes, 2 mM CaCl2 seemed to offer the same degree of stability to Ca-depleted BLA as that observed
with native BLA in the absence of 2 mM CaCl2. All these results suggest calcium-induced stabilization of BLA against GdnHCl denaturation. |
| first_indexed | 2024-03-06T07:28:28Z |
| format | Article |
| id | upm.eprints-13474 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:28:28Z |
| publishDate | 2010 |
| publisher | Academic Journals |
| record_format | dspace |
| spelling | upm.eprints-134742017-11-13T03:51:20Z http://psasir.upm.edu.my/id/eprint/13474/ Calcium-induced stabilization of α-amylase against guanidine hydrochloride denaturation Tan, Cheau Yuaan Raja Abdul Rahman, Raja Noor Zaliha Abdul Kadir, Habsah Tayya, Saad Guanidine hydrochloride (GdnHCl) denaturation of native and Ca-depleted Bacillus licheniformis α-amylase (BLA) was investigated both in the absence and presence of 2 mM calcium chloride (CaCl2)using circular dichroism, fluorescence spectroscopy and biological activity. In both states (Cadepleted and native form), the protein was denatured to a considerable extent in the absence of 2 mM CaCl2 with concomitant loss of biological activity upon increasing GdnHCl concentration. On the other hand, this effect was significantly reduced when 2 mM CaCl2 was included in the incubation mixture as revealed by a higher relative mean residue ellipticity, higher relative fluorescence intensity, smaller change in emission maximum and lesser reduction in biological activity. Interestingly, using these probes, 2 mM CaCl2 seemed to offer the same degree of stability to Ca-depleted BLA as that observed with native BLA in the absence of 2 mM CaCl2. All these results suggest calcium-induced stabilization of BLA against GdnHCl denaturation. Academic Journals 2010-11-15 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13474/1/13474.pdf Tan, Cheau Yuaan and Raja Abdul Rahman, Raja Noor Zaliha and Abdul Kadir, Habsah and Tayya, Saad (2010) Calcium-induced stabilization of α-amylase against guanidine hydrochloride denaturation. African Journal of Biotechnology, 9 (46). art. no. 9903FE419346. pp. 7934-7941. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/9903FE419346 |
| spellingShingle | Tan, Cheau Yuaan Raja Abdul Rahman, Raja Noor Zaliha Abdul Kadir, Habsah Tayya, Saad Calcium-induced stabilization of α-amylase against guanidine hydrochloride denaturation |
| title | Calcium-induced stabilization of α-amylase against guanidine hydrochloride denaturation |
| title_full | Calcium-induced stabilization of α-amylase against guanidine hydrochloride denaturation |
| title_fullStr | Calcium-induced stabilization of α-amylase against guanidine hydrochloride denaturation |
| title_full_unstemmed | Calcium-induced stabilization of α-amylase against guanidine hydrochloride denaturation |
| title_short | Calcium-induced stabilization of α-amylase against guanidine hydrochloride denaturation |
| title_sort | calcium induced stabilization of α amylase against guanidine hydrochloride denaturation |
| url | http://psasir.upm.edu.my/id/eprint/13474/1/13474.pdf |
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