Calcium-induced stabilization of α-amylase against guanidine hydrochloride denaturation

Calcium-induced stabilization of α-amylase against guanidine hydrochloride denaturation

Guanidine hydrochloride (GdnHCl) denaturation of native and Ca-depleted Bacillus licheniformis α-amylase (BLA) was investigated both in the absence and presence of 2 mM calcium chloride (CaCl2)using circular dichroism, fluorescence spectroscopy and biological activity. In both states (Cadepleted and...

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Bibliographic Details
Main Authors:	Tan, Cheau Yuaan, Raja Abdul Rahman, Raja Noor Zaliha, Abdul Kadir, Habsah, Tayya, Saad
Format:	Article
Language:	English
Published:	Academic Journals 2010
Online Access:	http://psasir.upm.edu.my/id/eprint/13474/1/13474.pdf

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