Cloning, extracellular expression and characterization of predominant β-CGTase from Bacillus sp. G1 in E. coli

Cloning, extracellular expression and characterization of predominant β-CGTase from Bacillus sp. G1 in E. coli

The cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) gene from Bacillus sp. G1 was successfully isolated and cloned into Escherichia coli. Analysis of the nucleotide sequence revealed the presence of an open reading frame of 2,109 bp and encoded a 674 amino acid protein. Purified CGTase exhibit...

Full description

Bibliographic Details
Main Authors: Ong, Rui Min, Goh, Kian Mau, Mahadi, Nor Muhammad, Hassan, Osman, Raja Abdul Rahman, Raja Noor Zaliha, Md. Illias, Rosli
Format: Article
Language:English
Published: Springer 2008
Online Access:http://psasir.upm.edu.my/id/eprint/13611/1/Cloning.pdf

Internet

http://psasir.upm.edu.my/id/eprint/13611/1/Cloning.pdf

Similar Items