Enhanced interferon-α2b production in periplasmic space of Escherichia coli through medium optimization using response surface method
The influence of different carbon and nitrogen sources on growth of recombinant Escherichia coli and human interferon-α2b (IFN-α2b) production in periplasmic space was studied in shake flask culture. A statistical method based on Plackett-Burman design was used to screen the main medium components t...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Bentham Open
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/14509/1/Enhanced%20interferon-%CE%B12b%20production%20in%20periplasmic%20space%20of%20Escherichia%20coli%20through%20medium%20optimization%20using%20response%20surface%20method.pdf |
| _version_ | 1796968659498827776 |
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| author | Tan, Joo Shun Ramanan, Ramakrishnan Nagasundara Azaman, Siti Nor Ani Ling, Tau Chuan Mustafa, Shuhaimi Ariff, Arbakariya |
| author_facet | Tan, Joo Shun Ramanan, Ramakrishnan Nagasundara Azaman, Siti Nor Ani Ling, Tau Chuan Mustafa, Shuhaimi Ariff, Arbakariya |
| author_sort | Tan, Joo Shun |
| collection | UPM |
| description | The influence of different carbon and nitrogen sources on growth of recombinant Escherichia coli and human interferon-α2b (IFN-α2b) production in periplasmic space was studied in shake flask culture. A statistical method based on Plackett-Burman design was used to screen the main medium components that greatly influenced the performance of the fermentation process. The optimization of medium was performed using response surface methodology (RSM) where three critical factors (glucose, yeast extract and peptone) were optimized using central composite design. The highest yield of periplasmic recombinant human interferon-α2b (PrIFN-α2b) (335.8 μg/L) was predicted to be obtained in optimized medium containing 5.47 g/L glucose, 55.24 g/L yeast extract and 42.27 g/L peptone. The production of IFN-α2b in periplasmic space in optimized medium was about 2.5, 11.7 and 124.4 times higher than Terrific broth (TB), Luria-Bertani (LB), and minimal medium (M9), respectively. |
| first_indexed | 2024-03-06T07:31:35Z |
| format | Article |
| id | upm.eprints-14509 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:31:35Z |
| publishDate | 2009 |
| publisher | Bentham Open |
| record_format | dspace |
| spelling | upm.eprints-145092019-04-08T08:51:01Z http://psasir.upm.edu.my/id/eprint/14509/ Enhanced interferon-α2b production in periplasmic space of Escherichia coli through medium optimization using response surface method Tan, Joo Shun Ramanan, Ramakrishnan Nagasundara Azaman, Siti Nor Ani Ling, Tau Chuan Mustafa, Shuhaimi Ariff, Arbakariya The influence of different carbon and nitrogen sources on growth of recombinant Escherichia coli and human interferon-α2b (IFN-α2b) production in periplasmic space was studied in shake flask culture. A statistical method based on Plackett-Burman design was used to screen the main medium components that greatly influenced the performance of the fermentation process. The optimization of medium was performed using response surface methodology (RSM) where three critical factors (glucose, yeast extract and peptone) were optimized using central composite design. The highest yield of periplasmic recombinant human interferon-α2b (PrIFN-α2b) (335.8 μg/L) was predicted to be obtained in optimized medium containing 5.47 g/L glucose, 55.24 g/L yeast extract and 42.27 g/L peptone. The production of IFN-α2b in periplasmic space in optimized medium was about 2.5, 11.7 and 124.4 times higher than Terrific broth (TB), Luria-Bertani (LB), and minimal medium (M9), respectively. Bentham Open 2009 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/14509/1/Enhanced%20interferon-%CE%B12b%20production%20in%20periplasmic%20space%20of%20Escherichia%20coli%20through%20medium%20optimization%20using%20response%20surface%20method.pdf Tan, Joo Shun and Ramanan, Ramakrishnan Nagasundara and Azaman, Siti Nor Ani and Ling, Tau Chuan and Mustafa, Shuhaimi and Ariff, Arbakariya (2009) Enhanced interferon-α2b production in periplasmic space of Escherichia coli through medium optimization using response surface method. The Open Biotechnology Journal, 3. pp. 117-124. ISSN 1874-0707 https://www.benthamopen.com/ABSTRACT/TOBIOTJ-3-117 10.2174/1874070700903010117 |
| spellingShingle | Tan, Joo Shun Ramanan, Ramakrishnan Nagasundara Azaman, Siti Nor Ani Ling, Tau Chuan Mustafa, Shuhaimi Ariff, Arbakariya Enhanced interferon-α2b production in periplasmic space of Escherichia coli through medium optimization using response surface method |
| title | Enhanced interferon-α2b production in periplasmic space of Escherichia coli through medium optimization using response surface method |
| title_full | Enhanced interferon-α2b production in periplasmic space of Escherichia coli through medium optimization using response surface method |
| title_fullStr | Enhanced interferon-α2b production in periplasmic space of Escherichia coli through medium optimization using response surface method |
| title_full_unstemmed | Enhanced interferon-α2b production in periplasmic space of Escherichia coli through medium optimization using response surface method |
| title_short | Enhanced interferon-α2b production in periplasmic space of Escherichia coli through medium optimization using response surface method |
| title_sort | enhanced interferon α2b production in periplasmic space of escherichia coli through medium optimization using response surface method |
| url | http://psasir.upm.edu.my/id/eprint/14509/1/Enhanced%20interferon-%CE%B12b%20production%20in%20periplasmic%20space%20of%20Escherichia%20coli%20through%20medium%20optimization%20using%20response%20surface%20method.pdf |
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