Optimisation of cryopreservation technique in Mokara golden nugget orchid using PVS2 vitrification.
The study was conducted to evaluate the effects of various sucrose concentrations on Mokara orchid shoots (0.5-1.0 and 1.0-1.5 cm) after precultured at 24 and 48 h. The 1.0-1.5 cm of Mokara shoots preculture at 0.25 M sucrose for 48 h was the best condition based on TTC assay and subsequently used f...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Academic Journals Inc.
2009
|