Simultaneous detection of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in sliced fruits using multiplex PCR.

This study aimed to determine the prevalence and quantity of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in sliced fruits from hawker stalls and hypermarkets in Malaysia. Analysis was carried out using the most probable number (MPN) – multiplex polymerase chain reaction (PCR) method...

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Main Authors: Chai, Fung Pui, Woan, Chwen Wong, Chai, Lay Ching, Nillian, Elexson, Mohamad Ghazali, Farinazleen, Cheah, Yoke Kqueen, Nakaguchi, Yoshitsugu, Nishibuchi, Mitsuaki, Radu, Son
Format: Article
Language:English
English
Published: Elsevier 2011
Subjects:
Online Access:http://psasir.upm.edu.my/id/eprint/16726/1/Simultaneous%20detection%20of%20Salmonella%20spp.pdf
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author Chai, Fung Pui
Woan, Chwen Wong
Chai, Lay Ching
Nillian, Elexson
Mohamad Ghazali, Farinazleen
Cheah, Yoke Kqueen
Nakaguchi, Yoshitsugu
Nishibuchi, Mitsuaki
Radu, Son
author_facet Chai, Fung Pui
Woan, Chwen Wong
Chai, Lay Ching
Nillian, Elexson
Mohamad Ghazali, Farinazleen
Cheah, Yoke Kqueen
Nakaguchi, Yoshitsugu
Nishibuchi, Mitsuaki
Radu, Son
author_sort Chai, Fung Pui
collection UPM
description This study aimed to determine the prevalence and quantity of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in sliced fruits from hawker stalls and hypermarkets in Malaysia. Analysis was carried out using the most probable number (MPN) – multiplex polymerase chain reaction (PCR) method. The prevalence of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in 210 samples of sliced fruits examined were 23.3%, 7.6% and 3.8%, respectively with estimated quantity varying from 0 to 19 MPN/g. This study urged the authority to look into the biosafety of sliced fruits in Malaysia.
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spelling upm.eprints-167262015-10-16T08:47:28Z http://psasir.upm.edu.my/id/eprint/16726/ Simultaneous detection of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in sliced fruits using multiplex PCR. Chai, Fung Pui Woan, Chwen Wong Chai, Lay Ching Nillian, Elexson Mohamad Ghazali, Farinazleen Cheah, Yoke Kqueen Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son This study aimed to determine the prevalence and quantity of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in sliced fruits from hawker stalls and hypermarkets in Malaysia. Analysis was carried out using the most probable number (MPN) – multiplex polymerase chain reaction (PCR) method. The prevalence of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in 210 samples of sliced fruits examined were 23.3%, 7.6% and 3.8%, respectively with estimated quantity varying from 0 to 19 MPN/g. This study urged the authority to look into the biosafety of sliced fruits in Malaysia. Elsevier 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16726/1/Simultaneous%20detection%20of%20Salmonella%20spp.pdf Chai, Fung Pui and Woan, Chwen Wong and Chai, Lay Ching and Nillian, Elexson and Mohamad Ghazali, Farinazleen and Cheah, Yoke Kqueen and Nakaguchi, Yoshitsugu and Nishibuchi, Mitsuaki and Radu, Son (2011) Simultaneous detection of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in sliced fruits using multiplex PCR. Food Control, 22 (2). pp. 337-342. ISSN 0956-7135 Salmonella food poisoning Foodborne diseases - Prevention Food - Microbiology 10.1016/j.foodcont.2010.05.021 English
spellingShingle Salmonella food poisoning
Foodborne diseases - Prevention
Food - Microbiology
Chai, Fung Pui
Woan, Chwen Wong
Chai, Lay Ching
Nillian, Elexson
Mohamad Ghazali, Farinazleen
Cheah, Yoke Kqueen
Nakaguchi, Yoshitsugu
Nishibuchi, Mitsuaki
Radu, Son
Simultaneous detection of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in sliced fruits using multiplex PCR.
title Simultaneous detection of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in sliced fruits using multiplex PCR.
title_full Simultaneous detection of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in sliced fruits using multiplex PCR.
title_fullStr Simultaneous detection of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in sliced fruits using multiplex PCR.
title_full_unstemmed Simultaneous detection of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in sliced fruits using multiplex PCR.
title_short Simultaneous detection of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in sliced fruits using multiplex PCR.
title_sort simultaneous detection of salmonella spp salmonella typhi and salmonella typhimurium in sliced fruits using multiplex pcr
topic Salmonella food poisoning
Foodborne diseases - Prevention
Food - Microbiology
url http://psasir.upm.edu.my/id/eprint/16726/1/Simultaneous%20detection%20of%20Salmonella%20spp.pdf
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