Simultaneous detection of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in sliced fruits using multiplex PCR.
This study aimed to determine the prevalence and quantity of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in sliced fruits from hawker stalls and hypermarkets in Malaysia. Analysis was carried out using the most probable number (MPN) – multiplex polymerase chain reaction (PCR) method...
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Format: | Article |
Language: | English English |
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Elsevier
2011
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Online Access: | http://psasir.upm.edu.my/id/eprint/16726/1/Simultaneous%20detection%20of%20Salmonella%20spp.pdf |
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author | Chai, Fung Pui Woan, Chwen Wong Chai, Lay Ching Nillian, Elexson Mohamad Ghazali, Farinazleen Cheah, Yoke Kqueen Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son |
author_facet | Chai, Fung Pui Woan, Chwen Wong Chai, Lay Ching Nillian, Elexson Mohamad Ghazali, Farinazleen Cheah, Yoke Kqueen Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son |
author_sort | Chai, Fung Pui |
collection | UPM |
description | This study aimed to determine the prevalence and quantity of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in sliced fruits from hawker stalls and hypermarkets in Malaysia. Analysis was carried out using the most probable number (MPN) – multiplex polymerase chain reaction (PCR) method. The prevalence of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in 210 samples of sliced fruits examined were 23.3%, 7.6% and 3.8%, respectively with estimated quantity varying from 0 to 19 MPN/g. This study urged the authority to look into the biosafety of sliced fruits in Malaysia. |
first_indexed | 2024-03-06T07:38:16Z |
format | Article |
id | upm.eprints-16726 |
institution | Universiti Putra Malaysia |
language | English English |
last_indexed | 2024-03-06T07:38:16Z |
publishDate | 2011 |
publisher | Elsevier |
record_format | dspace |
spelling | upm.eprints-167262015-10-16T08:47:28Z http://psasir.upm.edu.my/id/eprint/16726/ Simultaneous detection of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in sliced fruits using multiplex PCR. Chai, Fung Pui Woan, Chwen Wong Chai, Lay Ching Nillian, Elexson Mohamad Ghazali, Farinazleen Cheah, Yoke Kqueen Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son This study aimed to determine the prevalence and quantity of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in sliced fruits from hawker stalls and hypermarkets in Malaysia. Analysis was carried out using the most probable number (MPN) – multiplex polymerase chain reaction (PCR) method. The prevalence of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in 210 samples of sliced fruits examined were 23.3%, 7.6% and 3.8%, respectively with estimated quantity varying from 0 to 19 MPN/g. This study urged the authority to look into the biosafety of sliced fruits in Malaysia. Elsevier 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16726/1/Simultaneous%20detection%20of%20Salmonella%20spp.pdf Chai, Fung Pui and Woan, Chwen Wong and Chai, Lay Ching and Nillian, Elexson and Mohamad Ghazali, Farinazleen and Cheah, Yoke Kqueen and Nakaguchi, Yoshitsugu and Nishibuchi, Mitsuaki and Radu, Son (2011) Simultaneous detection of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in sliced fruits using multiplex PCR. Food Control, 22 (2). pp. 337-342. ISSN 0956-7135 Salmonella food poisoning Foodborne diseases - Prevention Food - Microbiology 10.1016/j.foodcont.2010.05.021 English |
spellingShingle | Salmonella food poisoning Foodborne diseases - Prevention Food - Microbiology Chai, Fung Pui Woan, Chwen Wong Chai, Lay Ching Nillian, Elexson Mohamad Ghazali, Farinazleen Cheah, Yoke Kqueen Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son Simultaneous detection of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in sliced fruits using multiplex PCR. |
title | Simultaneous detection of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in sliced fruits using multiplex PCR. |
title_full | Simultaneous detection of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in sliced fruits using multiplex PCR. |
title_fullStr | Simultaneous detection of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in sliced fruits using multiplex PCR. |
title_full_unstemmed | Simultaneous detection of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in sliced fruits using multiplex PCR. |
title_short | Simultaneous detection of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in sliced fruits using multiplex PCR. |
title_sort | simultaneous detection of salmonella spp salmonella typhi and salmonella typhimurium in sliced fruits using multiplex pcr |
topic | Salmonella food poisoning Foodborne diseases - Prevention Food - Microbiology |
url | http://psasir.upm.edu.my/id/eprint/16726/1/Simultaneous%20detection%20of%20Salmonella%20spp.pdf |
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