Single-step purification of the recombinant green fluorescent protein from intact Escherichia coli cells using preparative PAGE
Mechanical and non-mechanical breakages of bacterial cells are usually the preliminary steps in intracellular protein purification. In this study, the recombinant green fluorescent protein (GFP) was purified from intact Escherichia coli cells using preparative PAGE. In this purification process, cel...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/16730/1/Single-step%20purification%20of%20the%20recombinant%20.pdf |