Removal of poly-histidine fusion tags from recombinant proteins purified by expanded bed adsorption
Enzymatic methods have been used to cleave the C- or N-terminus polyhistidine tags from histidine tagged proteins following expanded bed purification using immobilized metal affinitychromatography (IMAC). This study assesses the use of Factor Xa and a genetically engineered exopeptidase dipeptidyl a...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley Periodicals
2005
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| Online Access: | http://psasir.upm.edu.my/id/eprint/18361/1/Removal%20of%20poly.pdf |