Microbial characterization of hydrogen producing bacteria in fermented food waste at different pH values
An anaerobic fermentation of food waste was conducted in a 0.5 L bioreactor incubated at a thermophilic temperature of 55 °C to evaluate the effects of different controlled pH values (5.0, 5.5 and 6.0) on biohydrogen production. Effective biohydrogen production was found at controlled pH 5.5 and 6.0...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/22362/1/Microbial%20characterization%20of%20hydrogen%20producing%20bacteria%20in%20fermented%20food%20waste%20at%20different%20pH%20values.pdf |
| _version_ | 1796970146541076480 |
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| author | Mohd Yasin, Nazlina Haiza Abdul Rahman, Nor'aini Che Man, Hasfalina Mohd Yusoff, Mohd Zulkhairi Hassan, Mohd Ali |
| author_facet | Mohd Yasin, Nazlina Haiza Abdul Rahman, Nor'aini Che Man, Hasfalina Mohd Yusoff, Mohd Zulkhairi Hassan, Mohd Ali |
| author_sort | Mohd Yasin, Nazlina Haiza |
| collection | UPM |
| description | An anaerobic fermentation of food waste was conducted in a 0.5 L bioreactor incubated at a thermophilic temperature of 55 °C to evaluate the effects of different controlled pH values (5.0, 5.5 and 6.0) on biohydrogen production. Effective biohydrogen production was found at controlled pH 5.5 and 6.0 corresponding to lower lactic acid production compared to pH 5.0. It was demonstrated that biohydrogen production from food waste was pH-dependent with hydrogen yields of 79, 76 and 23 mmol H2/L-media/d for pH 5.5, 6.0 and 5.0, respectively. Specific microbial determination for Clostridium sp. and total bacteria quantification were carried out by the fluorescent in-situ hybridization (FISH) technique. The number of Clostridium sp. for acclimatized sludge, fermentation broth at pH 5.0, 5.5 and 6.0 were 2.9 × 108, 3.6 × 108, 7.8 × 108 and 5.4 × 108 cells/ml, respectively. The quantification analysis showed that 92% of the total bacteria belonged to Clostridium sp. from clusters I and XI from the sample at controlled pH 5.5. The denaturing gradient gel electrophoresis (DGGE) bands of the sample after heat-treatment, acclimatization and during fermentation indicated the presence of Bacteroidetes, Caloromator australicus sp. and Clostridium sp. |
| first_indexed | 2024-03-06T07:53:44Z |
| format | Article |
| id | upm.eprints-22362 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:53:44Z |
| publishDate | 2011 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-223622016-09-02T08:54:04Z http://psasir.upm.edu.my/id/eprint/22362/ Microbial characterization of hydrogen producing bacteria in fermented food waste at different pH values Mohd Yasin, Nazlina Haiza Abdul Rahman, Nor'aini Che Man, Hasfalina Mohd Yusoff, Mohd Zulkhairi Hassan, Mohd Ali An anaerobic fermentation of food waste was conducted in a 0.5 L bioreactor incubated at a thermophilic temperature of 55 °C to evaluate the effects of different controlled pH values (5.0, 5.5 and 6.0) on biohydrogen production. Effective biohydrogen production was found at controlled pH 5.5 and 6.0 corresponding to lower lactic acid production compared to pH 5.0. It was demonstrated that biohydrogen production from food waste was pH-dependent with hydrogen yields of 79, 76 and 23 mmol H2/L-media/d for pH 5.5, 6.0 and 5.0, respectively. Specific microbial determination for Clostridium sp. and total bacteria quantification were carried out by the fluorescent in-situ hybridization (FISH) technique. The number of Clostridium sp. for acclimatized sludge, fermentation broth at pH 5.0, 5.5 and 6.0 were 2.9 × 108, 3.6 × 108, 7.8 × 108 and 5.4 × 108 cells/ml, respectively. The quantification analysis showed that 92% of the total bacteria belonged to Clostridium sp. from clusters I and XI from the sample at controlled pH 5.5. The denaturing gradient gel electrophoresis (DGGE) bands of the sample after heat-treatment, acclimatization and during fermentation indicated the presence of Bacteroidetes, Caloromator australicus sp. and Clostridium sp. Elsevier 2011-08 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22362/1/Microbial%20characterization%20of%20hydrogen%20producing%20bacteria%20in%20fermented%20food%20waste%20at%20different%20pH%20values.pdf Mohd Yasin, Nazlina Haiza and Abdul Rahman, Nor'aini and Che Man, Hasfalina and Mohd Yusoff, Mohd Zulkhairi and Hassan, Mohd Ali (2011) Microbial characterization of hydrogen producing bacteria in fermented food waste at different pH values. International Journal of Hydrogen Energy, 36 (16). pp. 9571-9580. ISSN 0360-3199; ESSN: 1879-3487 10.1016/j.ijhydene.2011.05.048 |
| spellingShingle | Mohd Yasin, Nazlina Haiza Abdul Rahman, Nor'aini Che Man, Hasfalina Mohd Yusoff, Mohd Zulkhairi Hassan, Mohd Ali Microbial characterization of hydrogen producing bacteria in fermented food waste at different pH values |
| title | Microbial characterization of hydrogen producing bacteria in fermented food waste at different pH values |
| title_full | Microbial characterization of hydrogen producing bacteria in fermented food waste at different pH values |
| title_fullStr | Microbial characterization of hydrogen producing bacteria in fermented food waste at different pH values |
| title_full_unstemmed | Microbial characterization of hydrogen producing bacteria in fermented food waste at different pH values |
| title_short | Microbial characterization of hydrogen producing bacteria in fermented food waste at different pH values |
| title_sort | microbial characterization of hydrogen producing bacteria in fermented food waste at different ph values |
| url | http://psasir.upm.edu.my/id/eprint/22362/1/Microbial%20characterization%20of%20hydrogen%20producing%20bacteria%20in%20fermented%20food%20waste%20at%20different%20pH%20values.pdf |
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