Role of α-helical structure in organic solvent-activated homodimer of elastase strain K

Recombinant elastase strain K overexpressed from E. coli KRX/pCon2(3) was purified to homogeneity by a combination of hydrophobic interaction chromatography and ion exchange chromatography, with a final yield of 48% and a 25-fold increase in specific activity. The purified protein had exhibited a fi...

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Detalhes bibliográficos
Main Authors: Raja Abdul Rahman, Raja Noor Zaliha, Salleh, Abu Bakar, Basri, Mahiran, Wong, Chee Fah
Formato: Artigo
Idioma:English
Publicado em: MDPI AG 2011
Acesso em linha:http://psasir.upm.edu.my/id/eprint/22407/1/Role%20of%20%CE%B1-Helical%20Structure%20in%20Organic%20Solvent-Activated%20Homodimer%20of%20Elastase%20Strain%20K.pdf