Role of α-helical structure in organic solvent-activated homodimer of elastase strain K
Recombinant elastase strain K overexpressed from E. coli KRX/pCon2(3) was purified to homogeneity by a combination of hydrophobic interaction chromatography and ion exchange chromatography, with a final yield of 48% and a 25-fold increase in specific activity. The purified protein had exhibited a fi...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/22407/1/Role%20of%20%CE%B1-Helical%20Structure%20in%20Organic%20Solvent-Activated%20Homodimer%20of%20Elastase%20Strain%20K.pdf |