Molecular cloning and production of recombinant phytase from bacillus subtilis ASUIA243 in Pichia Pastoris.

Molecular cloning and production of recombinant phytase from bacillus subtilis ASUIA243 in Pichia Pastoris.

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Phytase gene obtained from Bacillus subtilis ASUIA243 was cloned into a medium vector and transformed into E. coli. Restriction enzyme digestion was conducted to get blunt-ended phytase gene and ligated into the Pichia expression vector, pPICZ�A. The recombinant vector, pPICZ�A-243HPp was then linea...

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Bibliographic Details
Main Authors: Mohd Dali , Nor Soleha, Nuge , Tamrin, Mahamad Maifiah, Mohd. Hafidz, Yusof, Faridah, Meor Hussin, Anis Shobirin, Abd. El Aziem, Farouk, Mohd. Salleh, Hamzah
Format: Article
Language:English
English
Published: 2011
Online Access:http://psasir.upm.edu.my/id/eprint/24206/1/Molecular%20cloning%20and%20production%20of%20recombinant%20phytase%20from%20bacillus%20subtilis%20ASUIA243%20in%20Pichia%20Pastoris.pdf

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http://psasir.upm.edu.my/id/eprint/24206/1/Molecular%20cloning%20and%20production%20of%20recombinant%20phytase%20from%20bacillus%20subtilis%20ASUIA243%20in%20Pichia%20Pastoris.pdf

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