Molecular cloning and production of recombinant phytase from bacillus subtilis ASUIA243 in Pichia Pastoris.
Phytase gene obtained from Bacillus subtilis ASUIA243 was cloned into a medium vector and transformed into E. coli. Restriction enzyme digestion was conducted to get blunt-ended phytase gene and ligated into the Pichia expression vector, pPICZ�A. The recombinant vector, pPICZ�A-243HPp was then linea...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/24206/1/Molecular%20cloning%20and%20production%20of%20recombinant%20phytase%20from%20bacillus%20subtilis%20ASUIA243%20in%20Pichia%20Pastoris.pdf |