a-Tocopherol, ascorbic acid and carotenoid content in Centella asiatica leaf tissue and callus cultures.
Green leafy vegetables constitute a major part of balanced diet and are good sources of minerals and vitamins. These beneficial effects are attributed to the presence of antioxidants. Antioxidants also contribute to the defence mechanisms against oxidative stress. Centella asiatica, which is locally...
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| Format: | Article |
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Universiti Putra Malaysia Press
2011
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| author | Kusnan, Misri Jali, Azman Mahmood, Maziah Yusuf, Norhayati Mohd Fadzillah, Nor'Aini |
| author_facet | Kusnan, Misri Jali, Azman Mahmood, Maziah Yusuf, Norhayati Mohd Fadzillah, Nor'Aini |
| author_sort | Kusnan, Misri |
| collection | UPM |
| description | Green leafy vegetables constitute a major part of balanced diet and are good sources of minerals and vitamins. These beneficial effects are attributed to the presence of antioxidants. Antioxidants also contribute to the defence mechanisms against oxidative stress. Centella asiatica, which is locally known as ‘pegaga,’ is claimed to be rich in natural antioxidative compounds. This study was conducted to determine the amount of ascorbic acid, α-tocopherol and carotenoid content in twelve accessions of C. asiatica (CA01 to CA12-comprises of ‘pegaga nyonya’, ‘pegaga kampung’ and ‘pegaga salad’) leaf tissues and callus cultures. The antioxidative constituents of C. asiatica in the leaf tissues and cultures were found to vary significantly between the accessions. In particular, CA03 leaves (‘pegaga salad’) exhibited the highest concentrations of ascorbic acid (95.86 ± 12.60 µg/g.fwt), whereas CA10 (‘pegaga nyonya’) produced the highest concentration of α-tocopherol (0.233 ± 0.029 µg/g.fwt) and carotenoids (36.55 ± 0.06 µg/g.fwt). The antioxidants studied were also successfully detected in the cultures of C. asiatica, with CA08 callus (‘pegaga kampung’) being dominant in ascorbic acid (167.21 ± 5.30 µg/g.fwt) and a-tocopherol (5.72 ± 0.29 µg/g.fwt), whereas CA12 callus (‘pegaga kampung’) had the highest carotenoid content (1.04 ± 0.07 µg/g.fwt). Meanwhile, the amount of non-enzymatic antioxidants (except for carotenoid content) was significantly higher in the cell cultures compared to the leaf tissues. The results indicated that CA03 and CA10 leaf tissues, as well as CA08 and CA12 calli were good sources of natural antioxidants compared to other accessions. |
| first_indexed | 2024-03-06T08:01:56Z |
| format | Article |
| id | upm.eprints-25134 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T08:01:56Z |
| publishDate | 2011 |
| publisher | Universiti Putra Malaysia Press |
| record_format | dspace |
| spelling | upm.eprints-251342015-09-29T02:08:40Z http://psasir.upm.edu.my/id/eprint/25134/ a-Tocopherol, ascorbic acid and carotenoid content in Centella asiatica leaf tissue and callus cultures. Kusnan, Misri Jali, Azman Mahmood, Maziah Yusuf, Norhayati Mohd Fadzillah, Nor'Aini Green leafy vegetables constitute a major part of balanced diet and are good sources of minerals and vitamins. These beneficial effects are attributed to the presence of antioxidants. Antioxidants also contribute to the defence mechanisms against oxidative stress. Centella asiatica, which is locally known as ‘pegaga,’ is claimed to be rich in natural antioxidative compounds. This study was conducted to determine the amount of ascorbic acid, α-tocopherol and carotenoid content in twelve accessions of C. asiatica (CA01 to CA12-comprises of ‘pegaga nyonya’, ‘pegaga kampung’ and ‘pegaga salad’) leaf tissues and callus cultures. The antioxidative constituents of C. asiatica in the leaf tissues and cultures were found to vary significantly between the accessions. In particular, CA03 leaves (‘pegaga salad’) exhibited the highest concentrations of ascorbic acid (95.86 ± 12.60 µg/g.fwt), whereas CA10 (‘pegaga nyonya’) produced the highest concentration of α-tocopherol (0.233 ± 0.029 µg/g.fwt) and carotenoids (36.55 ± 0.06 µg/g.fwt). The antioxidants studied were also successfully detected in the cultures of C. asiatica, with CA08 callus (‘pegaga kampung’) being dominant in ascorbic acid (167.21 ± 5.30 µg/g.fwt) and a-tocopherol (5.72 ± 0.29 µg/g.fwt), whereas CA12 callus (‘pegaga kampung’) had the highest carotenoid content (1.04 ± 0.07 µg/g.fwt). Meanwhile, the amount of non-enzymatic antioxidants (except for carotenoid content) was significantly higher in the cell cultures compared to the leaf tissues. The results indicated that CA03 and CA10 leaf tissues, as well as CA08 and CA12 calli were good sources of natural antioxidants compared to other accessions. Universiti Putra Malaysia Press 2011 Article PeerReviewed Kusnan, Misri and Jali, Azman and Mahmood, Maziah and Yusuf, Norhayati and Mohd Fadzillah, Nor'Aini (2011) a-Tocopherol, ascorbic acid and carotenoid content in Centella asiatica leaf tissue and callus cultures. Pertanika Journal of Tropical Agricultural Science, 32 (2). pp. 331-339. ISSN 1511-3701 http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2034%20(2)%20Aug.%202011/19%20Pg%20331-339.pdf |
| spellingShingle | Kusnan, Misri Jali, Azman Mahmood, Maziah Yusuf, Norhayati Mohd Fadzillah, Nor'Aini a-Tocopherol, ascorbic acid and carotenoid content in Centella asiatica leaf tissue and callus cultures. |
| title | a-Tocopherol, ascorbic acid and carotenoid content in Centella asiatica leaf tissue and callus cultures. |
| title_full | a-Tocopherol, ascorbic acid and carotenoid content in Centella asiatica leaf tissue and callus cultures. |
| title_fullStr | a-Tocopherol, ascorbic acid and carotenoid content in Centella asiatica leaf tissue and callus cultures. |
| title_full_unstemmed | a-Tocopherol, ascorbic acid and carotenoid content in Centella asiatica leaf tissue and callus cultures. |
| title_short | a-Tocopherol, ascorbic acid and carotenoid content in Centella asiatica leaf tissue and callus cultures. |
| title_sort | a tocopherol ascorbic acid and carotenoid content in centella asiatica leaf tissue and callus cultures |
| work_keys_str_mv | AT kusnanmisri atocopherolascorbicacidandcarotenoidcontentincentellaasiaticaleaftissueandcalluscultures AT jaliazman atocopherolascorbicacidandcarotenoidcontentincentellaasiaticaleaftissueandcalluscultures AT mahmoodmaziah atocopherolascorbicacidandcarotenoidcontentincentellaasiaticaleaftissueandcalluscultures AT yusufnorhayati atocopherolascorbicacidandcarotenoidcontentincentellaasiaticaleaftissueandcalluscultures AT mohdfadzillahnoraini atocopherolascorbicacidandcarotenoidcontentincentellaasiaticaleaftissueandcalluscultures |