Verification of X- and Y-spermatozoa separation by nested polymerase chain reaction (PCR), motility and membrane integrity in bovine
The aim of the present study was to verify the presence of X- and Y-chromosome spermatozoa after separation with swimming speed using oestrus cows vagina mucus, Percoll discontinuous gradient (45 to 90%) and swim-up using TALP medium. The nested polymerase chain reaction (PCR) was used to determine...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Academic Journals
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/25345/1/25345.pdf |