Modified silver staining in 2DE improves protein detection even at extremely low sample concentration

The typical concentration of protein loaded varies from 0.13 to 1.40 μg/μL for a classical silver staining method in 2DE gel. Here, we present a simple modified classical silver staining method by modifying the silver impregnation and development reaction steps. This modified method detects the prot...

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Bibliographic Details
Main Authors: Liew, Yun Khoon, Neela, Vasantha Kumari, Awang Hamat, Rukman, Amin Nordin, Syafinaz, Chong, Pei Pei
Format: Article
Published: Wiley-VCH Verlag 2013