Native agarose gel electrophoresis and electroelution: a fast and cost-effective method to separate the small and large hepatitis B capsids

Hepatitis B core antigen (HBcAg) expressed in Escherichia coli is able to self-assemble into large and small capsids comprising 240 (triangulation number T = 4) and 180 (triangulation number T = 3) subunits, respectively. Conventionally, sucrose density gradient ultracentrifugation and SEC have been...

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Bibliographic Details
Main Authors: Yoon, Kam Yee, Tan, Wen Siang, Tey, Beng Ti, Lee, Khai Wooi, Ho, Kok Lian
Format: Article
Published: Wiley-VCH Verlag 2013