Rapid detection and identification of pathogens in patients with continuous ambulatory peritoneal dialysis (CAPD) associated peritonitis by 16s rRNA gene sequencing

Peritonitis still remains a serious complication with high rate of morbidity and mortality in patients on CAPD. Rapid and accurate identification of pathogens causing peritonitis in a CAPD patient is essential for early and optimal treatment. The aim of this study was to use 16S rRNA and ITS gene se...

Full description

Bibliographic Details
Main Authors: Ahmadi, S. H., Neela, Vasantha Kumari, Awang Hamat, Rukman, Goh, Bak Leong, Amin Nordin, Syafinaz
Format: Article
Language:English
Published: Malaysian Society of Parasitology and Tropical Medicine 2013
Online Access:http://psasir.upm.edu.my/id/eprint/29717/1/Rapid%20detection%20and%20identification%20of%20pathogens%20in%20patients%20with%20continuous%20ambulatory%20peritoneal%20dialysis.pdf
_version_ 1796971596079955968
author Ahmadi, S. H.
Neela, Vasantha Kumari
Awang Hamat, Rukman
Goh, Bak Leong
Amin Nordin, Syafinaz
author_facet Ahmadi, S. H.
Neela, Vasantha Kumari
Awang Hamat, Rukman
Goh, Bak Leong
Amin Nordin, Syafinaz
author_sort Ahmadi, S. H.
collection UPM
description Peritonitis still remains a serious complication with high rate of morbidity and mortality in patients on CAPD. Rapid and accurate identification of pathogens causing peritonitis in a CAPD patient is essential for early and optimal treatment. The aim of this study was to use 16S rRNA and ITS gene sequencing to identify common bacterial and fungal pathogens directly from the peritoneal fluid without culturing. Ninety one peritoneal fluids obtained from 91 different patients on CAPD suspected for peritonitis were investigated for etiological agents by 16S rRNA and ITS gene sequencing. Data obtained by molecular method was compared with the results obtained by culture method. Among the 45 patients confirmed for peritonitis based on international society of peritoneal dialysis (ISPD) guidelines, the etiological agents were identified in 37(82.2%) samples by culture method, while molecular method identified the etiological agents in 40(88.9%) samples. Despite the high potential application of the 16S rRNA and ITS gene sequencing in comparison to culture method to detect the vast majority of etiological agents directly from peritoneal fluids; it could not be used as a standalone test as it lacks sensitivity to identify some bacterial species due to high genetic similarity in some cases and inadequate database in Gene Bank. However, it could be used as a supplementary test to the culture method especially in the diagnosis of culture negative peritonitis.
first_indexed 2024-03-06T08:15:20Z
format Article
id upm.eprints-29717
institution Universiti Putra Malaysia
language English
last_indexed 2024-03-06T08:15:20Z
publishDate 2013
publisher Malaysian Society of Parasitology and Tropical Medicine
record_format dspace
spelling upm.eprints-297172015-09-18T03:15:35Z http://psasir.upm.edu.my/id/eprint/29717/ Rapid detection and identification of pathogens in patients with continuous ambulatory peritoneal dialysis (CAPD) associated peritonitis by 16s rRNA gene sequencing Ahmadi, S. H. Neela, Vasantha Kumari Awang Hamat, Rukman Goh, Bak Leong Amin Nordin, Syafinaz Peritonitis still remains a serious complication with high rate of morbidity and mortality in patients on CAPD. Rapid and accurate identification of pathogens causing peritonitis in a CAPD patient is essential for early and optimal treatment. The aim of this study was to use 16S rRNA and ITS gene sequencing to identify common bacterial and fungal pathogens directly from the peritoneal fluid without culturing. Ninety one peritoneal fluids obtained from 91 different patients on CAPD suspected for peritonitis were investigated for etiological agents by 16S rRNA and ITS gene sequencing. Data obtained by molecular method was compared with the results obtained by culture method. Among the 45 patients confirmed for peritonitis based on international society of peritoneal dialysis (ISPD) guidelines, the etiological agents were identified in 37(82.2%) samples by culture method, while molecular method identified the etiological agents in 40(88.9%) samples. Despite the high potential application of the 16S rRNA and ITS gene sequencing in comparison to culture method to detect the vast majority of etiological agents directly from peritoneal fluids; it could not be used as a standalone test as it lacks sensitivity to identify some bacterial species due to high genetic similarity in some cases and inadequate database in Gene Bank. However, it could be used as a supplementary test to the culture method especially in the diagnosis of culture negative peritonitis. Malaysian Society of Parasitology and Tropical Medicine 2013 Article NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29717/1/Rapid%20detection%20and%20identification%20of%20pathogens%20in%20patients%20with%20continuous%20ambulatory%20peritoneal%20dialysis.pdf Ahmadi, S. H. and Neela, Vasantha Kumari and Awang Hamat, Rukman and Goh, Bak Leong and Amin Nordin, Syafinaz (2013) Rapid detection and identification of pathogens in patients with continuous ambulatory peritoneal dialysis (CAPD) associated peritonitis by 16s rRNA gene sequencing. Tropical Biomedicine, 30 (4). pp. 602-607. ISSN 0127-5720 http://www.msptm.org/journal28.html
spellingShingle Ahmadi, S. H.
Neela, Vasantha Kumari
Awang Hamat, Rukman
Goh, Bak Leong
Amin Nordin, Syafinaz
Rapid detection and identification of pathogens in patients with continuous ambulatory peritoneal dialysis (CAPD) associated peritonitis by 16s rRNA gene sequencing
title Rapid detection and identification of pathogens in patients with continuous ambulatory peritoneal dialysis (CAPD) associated peritonitis by 16s rRNA gene sequencing
title_full Rapid detection and identification of pathogens in patients with continuous ambulatory peritoneal dialysis (CAPD) associated peritonitis by 16s rRNA gene sequencing
title_fullStr Rapid detection and identification of pathogens in patients with continuous ambulatory peritoneal dialysis (CAPD) associated peritonitis by 16s rRNA gene sequencing
title_full_unstemmed Rapid detection and identification of pathogens in patients with continuous ambulatory peritoneal dialysis (CAPD) associated peritonitis by 16s rRNA gene sequencing
title_short Rapid detection and identification of pathogens in patients with continuous ambulatory peritoneal dialysis (CAPD) associated peritonitis by 16s rRNA gene sequencing
title_sort rapid detection and identification of pathogens in patients with continuous ambulatory peritoneal dialysis capd associated peritonitis by 16s rrna gene sequencing
url http://psasir.upm.edu.my/id/eprint/29717/1/Rapid%20detection%20and%20identification%20of%20pathogens%20in%20patients%20with%20continuous%20ambulatory%20peritoneal%20dialysis.pdf
work_keys_str_mv AT ahmadish rapiddetectionandidentificationofpathogensinpatientswithcontinuousambulatoryperitonealdialysiscapdassociatedperitonitisby16srrnagenesequencing
AT neelavasanthakumari rapiddetectionandidentificationofpathogensinpatientswithcontinuousambulatoryperitonealdialysiscapdassociatedperitonitisby16srrnagenesequencing
AT awanghamatrukman rapiddetectionandidentificationofpathogensinpatientswithcontinuousambulatoryperitonealdialysiscapdassociatedperitonitisby16srrnagenesequencing
AT gohbakleong rapiddetectionandidentificationofpathogensinpatientswithcontinuousambulatoryperitonealdialysiscapdassociatedperitonitisby16srrnagenesequencing
AT aminnordinsyafinaz rapiddetectionandidentificationofpathogensinpatientswithcontinuousambulatoryperitonealdialysiscapdassociatedperitonitisby16srrnagenesequencing