Quantification of Escherichia coli O157:H7 in organic vegetables and chickens.
The organic foods’ market is becoming one of the rapidly growing sections in agricultural economies in the world. During the last two decades, food-borne outbreaks associated with fresh produce have rapidly increased. E. coli O57:H7, the caustic agent of acute hemorrhagic diarrhea and abdominal...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Faculty of Food Science and Technology, Universiti Putra Malaysia
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/30512/1/30512.pdf http://psasir.upm.edu.my/id/eprint/30512/7/Quantification%20of%20Escherichia%20coli%20O157.pdf |
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| author | Chang, Wei San Afsah Hejri, Leili Rukayadi, Yaya Khatib, Alfi Lye, Ying Ling Loo, Yuet Ying Mohd Shahril, Najwa Soopna, Puspanadan Kuan, Chee Hao Goh, Sur Guat Tang, John Yew Huat Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son |
| author_facet | Chang, Wei San Afsah Hejri, Leili Rukayadi, Yaya Khatib, Alfi Lye, Ying Ling Loo, Yuet Ying Mohd Shahril, Najwa Soopna, Puspanadan Kuan, Chee Hao Goh, Sur Guat Tang, John Yew Huat Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son |
| author_sort | Chang, Wei San |
| collection | UPM |
| description | The organic foods’ market is becoming one of the rapidly growing sections in agricultural
economies in the world. During the last two decades, food-borne outbreaks associated with
fresh produce have rapidly increased.
E. coli
O57:H7, the caustic agent of acute hemorrhagic
diarrhea and abdominal cramps, is mainly associated with meat and poultry product outbreaks
but frequent outbreaks linked to the consumption of vegetables have been reported. The aim of
this study was to investigate prevalence of
E. coli
O157:H7 in some organic foods. A total of 230
organic food samples including four-winged bean, tomato, white radish, red cabbage, chinese
cabbage, lettuce, cucumber and chicken form retailed groceries and supermarkets in Malaysia
were investigated. Low prevalence of
E. coli
O157:H7 was detected in organic vegetables and
chickens. The estimated quantity of
E. coli
O157:H7 in all samples ranged from <3 to >2400
MPN/g. The overall MPN/g estimate of
E. coli
O157:H7 in the samples from organic groceries
was higher than supermarket with the maximum of >2400 MPN/g. Most of the samples from
supermarket showed a minimum of <3 MPN/g. The specific target genes produced amplicons
of 259 bp and 625 bp after PCR amplification and
E. coli
O157:H7 was detected in 5.2% of
total organic samples. Prevalence of
E. coli
O157: H7 in organic foods from groceries (8.8%)
was particularly higher than supermarkets (1.0%). The highest prevalence of
E. coli
O157:H7
was observed in organic chickens (40%) purchased from groceries followed by four-winged
bean (10%) and white radish (3.3%). |
| first_indexed | 2024-03-06T08:17:47Z |
| format | Article |
| id | upm.eprints-30512 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T08:17:47Z |
| publishDate | 2013 |
| publisher | Faculty of Food Science and Technology, Universiti Putra Malaysia |
| record_format | dspace |
| spelling | upm.eprints-305122015-09-15T01:25:46Z http://psasir.upm.edu.my/id/eprint/30512/ Quantification of Escherichia coli O157:H7 in organic vegetables and chickens. Chang, Wei San Afsah Hejri, Leili Rukayadi, Yaya Khatib, Alfi Lye, Ying Ling Loo, Yuet Ying Mohd Shahril, Najwa Soopna, Puspanadan Kuan, Chee Hao Goh, Sur Guat Tang, John Yew Huat Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son The organic foods’ market is becoming one of the rapidly growing sections in agricultural economies in the world. During the last two decades, food-borne outbreaks associated with fresh produce have rapidly increased. E. coli O57:H7, the caustic agent of acute hemorrhagic diarrhea and abdominal cramps, is mainly associated with meat and poultry product outbreaks but frequent outbreaks linked to the consumption of vegetables have been reported. The aim of this study was to investigate prevalence of E. coli O157:H7 in some organic foods. A total of 230 organic food samples including four-winged bean, tomato, white radish, red cabbage, chinese cabbage, lettuce, cucumber and chicken form retailed groceries and supermarkets in Malaysia were investigated. Low prevalence of E. coli O157:H7 was detected in organic vegetables and chickens. The estimated quantity of E. coli O157:H7 in all samples ranged from <3 to >2400 MPN/g. The overall MPN/g estimate of E. coli O157:H7 in the samples from organic groceries was higher than supermarket with the maximum of >2400 MPN/g. Most of the samples from supermarket showed a minimum of <3 MPN/g. The specific target genes produced amplicons of 259 bp and 625 bp after PCR amplification and E. coli O157:H7 was detected in 5.2% of total organic samples. Prevalence of E. coli O157: H7 in organic foods from groceries (8.8%) was particularly higher than supermarkets (1.0%). The highest prevalence of E. coli O157:H7 was observed in organic chickens (40%) purchased from groceries followed by four-winged bean (10%) and white radish (3.3%). Faculty of Food Science and Technology, Universiti Putra Malaysia 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/30512/1/30512.pdf application/pdf en http://psasir.upm.edu.my/id/eprint/30512/7/Quantification%20of%20Escherichia%20coli%20O157.pdf Chang, Wei San and Afsah Hejri, Leili and Rukayadi, Yaya and Khatib, Alfi and Lye, Ying Ling and Loo, Yuet Ying and Mohd Shahril, Najwa and Soopna, Puspanadan and Kuan, Chee Hao and Goh, Sur Guat and Tang, John Yew Huat and Nakaguchi, Yoshitsugu and Nishibuchi, Mitsuaki and Radu, Son (2013) Quantification of Escherichia coli O157:H7 in organic vegetables and chickens. International Food Research Journal, 20 (2). pp. 1023-1029. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/ifrj-2013-20-issue-2.html |
| spellingShingle | Chang, Wei San Afsah Hejri, Leili Rukayadi, Yaya Khatib, Alfi Lye, Ying Ling Loo, Yuet Ying Mohd Shahril, Najwa Soopna, Puspanadan Kuan, Chee Hao Goh, Sur Guat Tang, John Yew Huat Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son Quantification of Escherichia coli O157:H7 in organic vegetables and chickens. |
| title | Quantification of Escherichia coli O157:H7 in organic vegetables and chickens. |
| title_full | Quantification of Escherichia coli O157:H7 in organic vegetables and chickens. |
| title_fullStr | Quantification of Escherichia coli O157:H7 in organic vegetables and chickens. |
| title_full_unstemmed | Quantification of Escherichia coli O157:H7 in organic vegetables and chickens. |
| title_short | Quantification of Escherichia coli O157:H7 in organic vegetables and chickens. |
| title_sort | quantification of escherichia coli o157 h7 in organic vegetables and chickens |
| url | http://psasir.upm.edu.my/id/eprint/30512/1/30512.pdf http://psasir.upm.edu.my/id/eprint/30512/7/Quantification%20of%20Escherichia%20coli%20O157.pdf |
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