Isolation and selection of reference genes for Ganoderma boninense gene expression study using quantitative real-time PCR (qPCR)

Isolation and selection of reference genes for Ganoderma boninense gene expression study using quantitative real-time PCR (qPCR)

Quan­ti­ta­tive real-time PCR (qPCR) has become a favourite method for quan­tifi­ca­tion of mRNA tran­scripts. How­ever, sev­eral opti­mi­sa­tion steps must be per­formed to avoid mis­lead­ing qPCR results. One of the steps is selec­tion of ref­er­ence genes for nor­mal­i­sa­tion pur­pose and these...

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Bibliographic Details
Main Authors: Lim, Fook Hwa, Iskandar, Nor Fakhrana, Abdul Rasid, Omar, Abu Seman, Idris, Ghulam Kadir, Ahmad Parveez, Ho, Chai Ling, Shaharuddin, Noor Azmi
Format: Article
Published: Malaysian Palm Oil Board 2014

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