Purification and characterisation of a novel amylase enzyme from red pitaya (Hylocereus polyrhizus) peel
An amylase enzyme from pitaya peel was purified 234.2-folds with 72.1% recovery using ammonium sulphate precipitation, gel filtration and ion exchange chromatography. Gel filtration chromatography and SDS–PAGE revealed that the enzyme is monomeric with a molecular weight of 42.1 kDa. The apparent Km...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/35114/1/Purification%20and%20characterisation%20of%20a%20novel%20amylase%20enzyme%20from%20red%20pitaya%20%28Hylocereus%20polyrhizus%29%20peel.pdf |