Purification and characterisation of a novel amylase enzyme from red pitaya (Hylocereus polyrhizus) peel
An amylase enzyme from pitaya peel was purified 234.2-folds with 72.1% recovery using ammonium sulphate precipitation, gel filtration and ion exchange chromatography. Gel filtration chromatography and SDS–PAGE revealed that the enzyme is monomeric with a molecular weight of 42.1 kDa. The apparent Km...
Main Authors: | Amid, Mehrnoush, Abd Manap, Mohd Yazid |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2014
|
Online Access: | http://psasir.upm.edu.my/id/eprint/35114/1/Purification%20and%20characterisation%20of%20a%20novel%20amylase%20enzyme%20from%20red%20pitaya%20%28Hylocereus%20polyrhizus%29%20peel.pdf |
Similar Items
-
Microencapsulation of Purified Amylase Enzyme from Pitaya (Hylocereus polyrhizus) Peel in Arabic Gum-Chitosan using Freeze Drying
by: Mehrnoush Amid, et al.
Published: (2014-03-01) -
Microencapsulation of purified amylase enzyme from pitaya (Hylocereus polyrhizus) peel in Arabic gum-chitosan using freeze drying
by: Amid, Mehrnoush, et al.
Published: (2014) -
A Novel Aqueous Two Phase System Composed of a Thermo-Separating Polymer and an Organic Solvent for Purification of Thermo-Acidic Amylase Enzyme from Red Pitaya (Hylocereus polyrhizus) Peel
by: Mehrnoush Amid, et al.
Published: (2014-05-01) -
A novel aqueous two phase system composed of a thermo-separating polymer and an organic solvent for purification of thermo-acidic amylase enzyme from red pitaya (Hylocereus polyrhizus) peel
by: Amid, Mehrnoush, et al.
Published: (2014) -
Optimization of Extraction of Novel Pectinase Enzyme Discovered in Red Pitaya (Hylocereus polyrhizus) Peel
by: Nor Khanani Zohdi, et al.
Published: (2013-11-01)