Purification of glutathione S-transferase (GST) using mixed mode chromatography

A simple and small scale laboratory method to compare between ultrasonication, glass bead shaking and chemical lysis were evaluated for the release of recombinant Glutathione S-Transferase (GST) from Escherichia coli. Since the protein Glutathione S-Transferase is expressed intracellularly, cell dis...

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Bibliographic Details
Main Authors: M., Sivapragasam, Abdullah, Norhafizah
Format: Article
Published: ESRSA Publications 2014