Purification of glutathione S-transferase (GST) using mixed mode chromatography
A simple and small scale laboratory method to compare between ultrasonication, glass bead shaking and chemical lysis were evaluated for the release of recombinant Glutathione S-Transferase (GST) from Escherichia coli. Since the protein Glutathione S-Transferase is expressed intracellularly, cell dis...
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| Format: | Article |
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ESRSA Publications
2014
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| _version_ | 1825948583222837248 |
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| author | M., Sivapragasam Abdullah, Norhafizah |
| author_facet | M., Sivapragasam Abdullah, Norhafizah |
| author_sort | M., Sivapragasam |
| collection | UPM |
| description | A simple and small scale laboratory method to compare between ultrasonication, glass bead shaking and chemical lysis were evaluated for the release of recombinant Glutathione S-Transferase (GST) from Escherichia coli. Since the protein Glutathione S-Transferase is expressed intracellularly, cell disruption process is the precursor step for protein recovery.GST was purified using assessment with PPA and HEA HyperCel resin. Optimum release of GST was via ultrasonication, 70% amplitude size with enzyme release of 129.9 U/mL. Purification yields via PPA HyperCel yielded 96% recovery while purification using HEA HyperCel yielded a 93% enzyme recovery. |
| first_indexed | 2024-03-06T08:31:19Z |
| format | Article |
| id | upm.eprints-35117 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T08:31:19Z |
| publishDate | 2014 |
| publisher | ESRSA Publications |
| record_format | dspace |
| spelling | upm.eprints-351172015-12-30T10:58:20Z http://psasir.upm.edu.my/id/eprint/35117/ Purification of glutathione S-transferase (GST) using mixed mode chromatography M., Sivapragasam Abdullah, Norhafizah A simple and small scale laboratory method to compare between ultrasonication, glass bead shaking and chemical lysis were evaluated for the release of recombinant Glutathione S-Transferase (GST) from Escherichia coli. Since the protein Glutathione S-Transferase is expressed intracellularly, cell disruption process is the precursor step for protein recovery.GST was purified using assessment with PPA and HEA HyperCel resin. Optimum release of GST was via ultrasonication, 70% amplitude size with enzyme release of 129.9 U/mL. Purification yields via PPA HyperCel yielded 96% recovery while purification using HEA HyperCel yielded a 93% enzyme recovery. ESRSA Publications 2014-02 Article PeerReviewed M., Sivapragasam and Abdullah, Norhafizah (2014) Purification of glutathione S-transferase (GST) using mixed mode chromatography. International Journal of Engineering Research & Technology, 3 (2). pp. 1292-1299. ISSN 2278-0181 http://www.ijert.org/view-pdf/8150/purification-of-glutathione-s-transferase-gst-using-mixed-mode-chromatography |
| spellingShingle | M., Sivapragasam Abdullah, Norhafizah Purification of glutathione S-transferase (GST) using mixed mode chromatography |
| title | Purification of glutathione S-transferase (GST) using mixed mode chromatography |
| title_full | Purification of glutathione S-transferase (GST) using mixed mode chromatography |
| title_fullStr | Purification of glutathione S-transferase (GST) using mixed mode chromatography |
| title_full_unstemmed | Purification of glutathione S-transferase (GST) using mixed mode chromatography |
| title_short | Purification of glutathione S-transferase (GST) using mixed mode chromatography |
| title_sort | purification of glutathione s transferase gst using mixed mode chromatography |
| work_keys_str_mv | AT msivapragasam purificationofglutathionestransferasegstusingmixedmodechromatography AT abdullahnorhafizah purificationofglutathionestransferasegstusingmixedmodechromatography |